Detail of "80307-12-6"

Reference

Immobilization of active agents on hydroxyl group-carrying substrates with a silane and heterobifunctional crosslinking agent

Immobilization of active agents on hydroxyl group-carrying substrates with a silane and heterobifunctional crosslinking agent. Eigler, Frances S.; Georger, Jacque; Bhatia, Suresh K.; Calvert, Jeff; Shriver-Lake, Lisa C.; Bredehorst, Reinhard (USA ). U.S. US 5077210 A 31 Dec 1991,11 pp. (United States of America). CODEN: USXXAM. CLASS: ICM: C12N011-14. ICS: C12N011-04; G01N033-551; G01N033-549. NCL: 435176000. APPLICATION: US 89-297088 13 Jan 1989. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 15 Active agents such as proteins are covalently immobilized on substrates carrying OH groups by reacting the substrates with a silane and a heterobifunctional crosslinker. 4420-74-0 and 80307-12-6 which are cas registry numbers of chemicals are mentioned. Preferably, the silane has a functional group which reacts with the OH group of the substrate and a thiol terminal group which reacts with a functional group of the crosslinker. The crosslinking agent contains a succinimide group that reacts with an amino group of the active agent. Affinity-purified rabbit antigoat IgG was immobilized on glass coverslips and fused silica optical fibers treated with mercaptomethyldimethyloxysilane or mercaptopropyltrimethyloxysilane and N-g-maleimidobutyryloxysuccinimide ester. Antigen binding could be measured on coverslips or fibers exposed to antigen concns. 30.5 mg/mL. .