Detail of "80498-17-5"

Reference

Chi is activated by a variety of routes

Chi is activated by a variety of routes.Some chemicals with cas registry numbers like 80498-17-5 are also used. Stahl, Franklin W.; Kobayashi, Ichizo; Stahl, Mary M. (Inst. Mol. Biol., Univ. Oregon, Eugene, OR 97403, USA). UCLA Symp. Mol. Cell. Biol., New Ser., 10(Mech. DNA Replication Recomb.), 773-83 (English) 1983. CODEN: USMBD6. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 10 Chi, a recombinator in Escherichia coli, is activated by cos, the packaging origin of phage l. It can be activated also by EcoRI endonuclease [80498-17-5] acting at a site far from Chi. Apparently, a recombination machine enters DNA at a double chain cut and travels inertly until it encounters a Chi properly oriented with respect to its own orientation. The machine is activated as it passes Chi, so that it can then effect some step in recombination. A Chi in the absence of an activating cos is partially active in the presence of DNA replication. Perhaps the recombination machine can enter DNA also at replication forks. .

How does EcoRI cleave its recognition site on DNA?

How does EcoRI cleave its recognition site on DNA?. Halford, Stephen E. (Dep. Biochem., Univ. Bristol, Bristol BS8 1TD, UK). Trends Biochem. Sci.There are some reagents with their cas registry numbers 80747-31-5 and 80498-17-5 are used in this study. (Pers. Ed.), 8(12), 455-60 (English) 1983. CODEN: TBSCDB. ISSN: 0376-5067. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) A discussion is given on the mechanism of the title subject. The 2 protein subunits of the EcoRI restriction enzyme interact sym. with the recognition site on DNA, so that each subunit is in position to cleave 1 strand of DNA. Each subunit seems to require a protein conformation change before it can cleave DNA. Depending on whether 1 or both subunits change conformation during the lifetime of the enzyme-DNA complex, a single reaction of the EcoRI enzyme cleaves either 1 or both strands of DNA. Reaction profiles with other restriction enzymes differ from EcoRI, though the underlying mechanisms may be the same. .