Reference

Highly sensitive and specific radioimmunoassays for dihydroergotoxine components in plasma

Highly sensitive and specific radioimmunoassays for dihydroergotoxine components in plasma. Collignon, F.; Pradelles, P. (Inst. Biopharm., Rhone-Poulenc, Fr.).Chemicals with cas numbers 25447-65-8 and 17479-19-5 also play role. Eur. J. Nucl. Med., 9(1), 23-7 (English) 1984. CODEN: EJNMD9. ISSN: 0340-6997. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The development of 3 analogous radioimmunoassay procedures for dihydroergotoxine mesylate [8067-24-1] components is described. The antisera were produced by immunization of rabbits with immunogens obtained by coupling egg albumin to the indole group of each ergot alkaloid deriv. In each radioimmunoassay, antibodies do not cross-react more than 5% with the 2 other derivs. The tracers iodinated with 125I were prepd. by the chloramine-T method and purified by thin-layer chromatog. Both the high antibody affinity and high specific radioactivity of the tracers allow a sensitive assay (detection limit <20 pg/mL) in human plasma. After high-performance liq. chromatog. of the extd. plasma, no immunoreactive materials other than those corresponding to the elution of the 3 dihydroergotoxine components were detected. Two preliminary pharmacokinetic profiles obtained in dogs and humans for each deriv. are shown. .

Cerebral blood flow, cerebral metabolism and neurological deficit during an experimental infarct

Cerebral blood flow, cerebral metabolism and neurological deficit during an experimental infarct. Application to the study of a rye ergot derivative. Cahn, J.; Borzeix, M. G. (Dep. Ther. Exp., SIR Int., Montrouge F 92120, Fr.). Presse Med., 12(48), 3058-60 (French) 1983. CODEN: PRMEEM. ISSN: 0755-4982. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The cerebral edema, the fall in cerebral blood flow, and the changes in cerebral metab. in rats with multiple infarction (induced by intracarotid injection of Na arachidonate) were counteracted by the alkaloid hydergine (I) [8067-24-1](0.5 or 1 mg/kg, i.v.). 8067-24-1 which is the cas registry number of one of substances is just one of reagents here. This was true in rats with the addnl. risk factors of age and hypertension, as well as in young and normotensive rats. No data are given on the effects of I on the infarct-induced motor alterations (neurol. deficit). .