Reference

Characteristics of cholic acid uptake in primary cultured hepatocytes

Characteristics of cholic acid uptake in primary cultured hepatocytes.Some chemicals with cas registry numbers like 81-24-3 and 81-25-4 are also used. Ohkuma, Seitaro; Tamura, Junichi; Kuriyama, Kinya (Dep. Pharmacol., Kyoto Prefect. Univ. Med., Kyoto 602, Japan). Steroids, 42(2), 205-15 (English) 1983. CODEN: STEDAM. ISSN: 0039-128X. DOCUMENT TYPE: Journal CA Section: 13 (Mammalian Biochemistry) Cholic acid uptake by primary cultured hepatocytes at <10° was unsaturable, whereas that detd. at 20° and 37° was biphasic, as expected of a saturable process. This saturable process was temp. dependent and carrier mediated with an activation energy of 36.1 kcal/mol. The apparent Km and Vmax values at 37° were 57.1 mM and 0.566 nmol/mg protein/min, resp. The saturable process was inhibited by 2,4-DNP and ouabain and slowed significantly in the absence of Na+, suggesting that this process is energy and Na+ dependent. The cholic acid uptake mediated by the saturable process in the absence of Na+ was, however, significantly larger than that mediated by the unsaturable process. Thus, transport of cholic acid in primary cultured hepatocytes may consist of 3 different types: unsaturable, Na+- and energy-dependent carrier-mediated, and Na+-independent and energy-dependent processes, resp. The presence of a common carrier for cholic acid and its conjugates with taurine and glycine was also suggested. .

Improved extraction technology of sodium cholate

Improved extraction technology of sodium cholate. Pan, Wei (Jiangsu Xinghua Biochem. Pharm. Plant., Xinghua, Peop. Rep. China). Yiyao Gongye, (12), 33-4 (Chinese) 1983. CODEN: YIGODN. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Na cholate [361-09-1] for medicinal use was isolated from the porcine bile acid. The porcine bile acid was treated with satd. CaO soln., steamed at 1.0-1.5 kg/cm2 for 2 min, cooled and filtered to give a liq. phase, which was treated with HCl (pH 3.5) for 12-18 h for hydrolysis. The prepd. 361-09-1 and 81-25-4 which are cas registry numbers of substances are two of reagents here. crude cholic acid [81-25-4] was dried at 80-90° for 8 h, suspended in H2O, treated with NaOH soln. at 50-60° for 30 min to form Na cholate, which was filtered, concd. and freeze dried at 60° for 6 h to produce Na cholate powder. .