Detail of "82-94-0"

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The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA

The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA. Oud, P. S.; Henderik, J. B. J.; Huysmans, A. C. L. M.; Pahlplatz, M.Chemicals with cas numbers 82-94-0 and 9001-75-6 also play role. M. M.; Hermkens, H. G.; Tas, J.; James, J.; Vooijs, G. P. (Inst. Pathol. Anat., Univ. Nijmegen, Nijmegen NL-6525 GA, Neth.). Histochemistry, 80(1), 49-57 (English) 1984. CODEN: HCMYAL. ISSN: 0301-5564. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) The protein dyes Light Green and Orange II were studied sep. and in combination with the Feulgen-Pararosaniline (SO2) and -Thionin(SO2) method for the simultaneous detn. of DNA and protein. With polyacrylamide model films the pH dependency, specificity, and stoichiometry of Light Green and Orange II were investigated. The results of both staining methods with different biol. objects were compared. In addn., the Feulgen-Thionin(SO2) method was studied with model films with respect to its specificity and stoichiometry. In biol. objects, it was compared with the Feulgen-Pararosaniline (SO2) method. When combining the Light Green staining with the Feulgen-Pararosaniline (SO2) procedure and the Orange II staining with Feulgen-Thionin (SO2), both Feulgen-DNA stainings, which were first applied, proved to be unaffected by the following protein staining procedure. When the Feulgen procedure was carried out without the dye, followed by Light Green staining the latter became reduced when a sulfite water rinse was included but was unaffected when a running tap water rinse was used. In the case of the Orange II staining a serious redn. in dye binding capacity was found in both situations. When the Feulgen-Pararosaniline (SO2) Light Green procedure was done on isolated nuclei with all dyes present, a decrease of protein dye binding was obsd., similar to that found with the well-known Feulgen-Pararosaniline (SO2) Naphthol Yellow S combination. It is concluded that, in spite of this redn., the latter 2 combinations can be used for the cytophotometric anal. of DNA and protein in the same object. .

Coextraction in the chloroaurate anion-basic dye-organic solvent system

Coextraction in the chloroaurate anion-basic dye-organic solvent system. Tarayan, V. M.; Mikaelyan, D. A. (Erevan. Gos. Univ., Yerevan, USSR). Arm. 7440-57-5 and 82-94-0 are cas registry numbers. These chemicals are also mentioned in this article. Khim. Zh., 29(12), 1027-32 (Russian) 1976. CODEN: AYKZAN. DOCUMENT TYPE: Journal CA Section: 68 (Phase Equilibriums, Chemical Equilibriums, and Solutions) The extn. of AuCl4- by the basic dye methyl green was studied as a function of pH, methyl green concn., and type of diluent (C6H6, EtOAc, BuOAc, and CCl4). The Au3+ concn. in AuCl4- soln. was detd. by amperometric titrn. with Hg(NO3). The extd. species has .lambda.max. = 640 nm. The equil. with C6H6 the quantity of extd. Au is independent of pH; at pH = 1 the methyl green cation (MG); AuCl4- (MG)AuCl4.(MG)Cl. Due to the coextn. of simple (MG)Cl salt at pH = 1, the absorbance of org. phase is higher than when the aq. phase pH contains 2N HCl. Extn. with CCl4 leads to a 2-fold absorbance decrease, which indicates that no coextn. of the simple (MG) salt occurs. .