Detail of "827-61-2"

Reference

Effects of intracellular antibodies to cGMP on responses of cortical neurons of awake cats to extracellular application of muscarinic agonists

Effects of intracellular antibodies to cGMP on responses of cortical neurons of awake cats to extracellular application of muscarinic agonists. Swartz, Barbara E.; Woody, Charles D. (Brain Res. Inst., Univ. California, Los Angeles, CA 90024, USA). Exp. Neurol., 86(2), 388-404 (English) 1984. CODEN: EXNEAC. ISSN: 0014-4886. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Intracellular injection of specific antibody to cGMP [7665-99-8] selectively produced substantial decreases in input resistance (Rm) in neurons of the motor cortex that had responded with increased resistance to prior application of muscarinic agents. Intracellular injection of nonspecific Igs (IgG) did not produce this effect. Some nonspecific effects on spike prodn. did occur in cells given IgG or cGMP antibodies. The decrease in Rm may be consequential to a redn. in baseline amts. of active cGMP due to binding of cGMP with the injected antibody. In cells which demonstrated a prior increase in Rm following extracellular application of the muscarinic agonist, aceclidine [827-61-2], or acetylcholine [51-84-3], injection of antibody to cGMP suppressed the increase in Rm to subsequent applications of these muscarinic agents. Some increases in firing rate to these agents continued to be obsd. after injection of cGMP antibodies. CGMP may mediate effects of muscarinic neurotransmission on the conductances of neurons of the motor cortex of awake cats. Apparently, intracellular injection of antibodies to specific cellular elements is feasible in cortical neurons of awake cats and may be a useful adjunct to future studies of neurotransmitter mechanisms.

Comparative sensitivity of M-cholinergic receptors of various localizations to cholinotropic substances

Comparative sensitivity of M-cholinergic receptors of various localizations to cholinotropic substances. Angelova, L. A. (IMMI im. Sechenova, Moscow, USSR). Farmakol. Toksikol. (Moscow), 48(2), 115-20 (Russian) 1985. CODEN: FATOAO. ISSN: 0014-8318. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 2 The effects of acetylcholine [51-84-3], methacholine [55-92-5], carbacholine [51-83-2], aceclidine [827-61-2], pilocarpine [92-13-7], proserine [51-60-5], anatruxonium [35515-77-6], diazoline [6153-33-9], novocaine [51-05-8], and atropine [51-55-8] on isolated cat atria, fundus, artery, and bronchi prepns. were compared. Sensitivities of the 4 tissues to acetylcholine, methacholine, and carbacholine varied considerably. Aceclidine produced similar activity in all 4 tissues. Anatruxonium and diazoline most effectively antagonized the effect of carbacholine in atrial prepns. Novocaine more effectively antagonized the effect of carbacholine on atria and bronchi than on stomach.