Detail of "84982-60-5"

Reference

Methodology for the extraction and analysis of hydrocarbons and carboxylic acids in atmospheric particulate matter

Methodology for the extraction and analysis of hydrocarbons and carboxylic acids in atmospheric particulate matter. Barkenbus, B. D.; MacDougall, C. S.; Griest, W. H.; Caton, J. E. (Anal. Chem. Div., Oak Ridge Natl. Lab., Oak Ridge, TN 37830, USA). Atmos. Environ., 17(8), 1537-43 (English) 1983. CODEN: ATENBP. ISSN: 0004-6981. DOCUMENT TYPE: Journal CA Section: 59 (Air Pollution and Industrial Hygiene) Particulate org. atm. pollutants collected in ambient air sampling are quant. analyzed for specific aliph. hydrocarbons, and mono-, and dicarboxylic acids by gas chromatog. of trimethylsilyl-derivatized high-vol. air filter exts. Atm. particulate filters are Soxhlet-extd. for 24 h using the C6H6-MeOH azeotrope. Liq. scintillation spectrometric measurements of radio-labeled benzo[a]pyrene, dotriacontane [544-85-4], stearic acid [57-11-4], and succinic acid [110-15-6] tracers indicated near-quant. extn. recoveries. Palmitic acid-t [84982-60-5] and benzo[a]pyrene-14C [3107-03-7] were selected for routine monitoring of extn.In this experiment, several chemicals are used like 629-94-7 and 334-48-5 recoveries after the fidelity of the 3H-label during Soxhlet extn. was demonstrated. Trimethylsilyl-derivatization of an aliquot of the crude ext. allows carboxylic acids and paraffins to be detd. readily by gas chromatog. .

Radioisotopic method for the measurement of lipolysis in small samples of human adipose tissue

Radioisotopic method for the measurement of lipolysis in small samples of human adipose tissue. Leibel, Rudolph L.Several substances are used for example 89486-16-8 and 84982-60-5 which are their cas registry numbers.; Hirsch, Jules; Berry, Elliot M.; Gruen, Rhoda K. (Lab. Hum. Behav. Metab., Rockefeller Univ., New York, NY, USA). J. Lipid Res., 25(1), 49-57 (English) 1984. CODEN: JLPRAW. ISSN: 0022-2275. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 13 To facilitate the study of adrenoreceptor response in small needle biopsy samples of human s.c. adipose tissue, a dual radioisotopic technique was developed for measuring lipolysis rate. Aliquots (20-75 mg) of adipose tissue fragments were incubated in a buffered albumin medium contg. [3H]palmitate and [14C]glucose, each of high specific activity. In neutral glycerides synthesized in this system, [14C]glucose is incorporated exclusively into the glyceride-glycerol moiety and 3H appears solely in the esterified fatty acids. Alpha-2- and beta-1 adrenoreceptor activation of tissue incubated in this system does not alter rates of 14C-labeled glyceride accumulation, but does produce a resp. increase or decrease in the specific activity of fatty acids esterified into newly synthesized glycerides. This alteration in esterified fatty acid specific activity is reflected in the ratio of 14C:3H in newly synthesized triglycerides extd. from the incubated adipose tissue. There is a high correlation between the 14C:3H ratio in triglycerides and the rate of lipolysis as reflected in glycerol release into the incubation medium. The degree of adrenoreceptor activation by various concns. of lipolytic and anti-lipolytic substances can be assessed by comparing this ratio in stimulated tissue to that characterizing unstimulated tissue or in the incubation medium. This technique permits the study of very small, unweighed tissue biopsy fragments, the only limitation on sensitivity being the specific activity of the medium glucose and palmitate. It is, therefore, useful for serial examns. of adipose tissue adrenoreceptor dose-response characteristics under a variety of clin. circumstances. .