Detail of "85284-16-8"

Reference

Water soluble fluors

Water soluble fluors. Lee, Duk Hi; Feierberg, Susan; O'Brien, Robert E. (New England Nuclear Corp., USA). Eur. Pat. Appl. EP 87639 A2 7 Sep 1983, 41 pp. DESIGNATED STATES: R: AT, BE, CH, DE, FR, GB, LI, NL, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: G01T001-204; G01T001-29; C07D263-32. APPLICATION: EP 83-101287 10 Feb 1983. PRIORITY: US 82-352209 25 Feb 1982.There are some reagents with their cas registry numbers 85284-16-8 and 92-71-7 are used in this study. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 8, 28, 46, 71, 74, 79 New water-sol. fluors for enhancing autoradiog. or fluorog. images were prepd. which consist of a fluor dehydro deriv. bound to a surfactant moiety (which renders the mol. hydrophilic) via a chem. bond or other component (e.g., thio, carboxy, alkylene groups). For autoradiog., the absorbent or adsorbent is contacted (by immersion) for <2 h with at least 2 of the prepd. fluors (each having a quantum efficiency of ~0.6) prior to or after inclusion of the radioactive emitters in the medium, so that the mixt. contains 0.1-85% fluor, followed by drying and exposing a photo-sensitive film to the adsorbent or absorbent. The prepd. fluors are simpler and safer for use. Thus, for the prepn. of 4-chloromethyl-2,5-diphenyloxazole, 100 g isonitrosopropiophenone and ~65 g benzaldehyde were dissolved in glacial HOAc, HCl gas was bubbled through until a yellow ppt. formed, and the ppt. was dissolved in MeOH, neutralized, dissolved in EtOH, and hydrogenated with Raney-Ni catalyst for ~2 h to give 4-methyl-2,5-diphenyloxazole which was chlorinated to give the desired compd. The prepd. fluors may be used for, e.g., the detection of 3H-DNA in electrophoretic gels. .