Reference

Radioimmunoassay of the anti-hypertensive agent debrisoquin

Radioimmunoassay of the anti-hypertensive agent debrisoquin. Dixon, Ross; Fahrenholtz, Kenneth E.; Burger, Walter; Perry, Clark (Res. Div., Hoffmann-La Roche, Inc., Nutley, N. J., USA). Res. Commun. Chem. Pathol. Pharmacol., 16(1), 121-9 (English) 1977. CODEN: RCOCB8. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Antiserums to debrisoquin sulfate (I) [581-88-4] a widely used antihypertensive agent, were obtained from rabbits following immunization with a conjugate of 4-[(1,2,3,4-tetrahydroisoquinolin-2-yl)(imino)-methylamino] butanoic acid and bovine serum albumin. Employing 3H-labeled I as the radioligand for the antiserum, a radioimmunoassay (RIA) was developed which allowed for the specific detn. of the drug directly in plasma. After logit transformation, a linear calibration line was obtained between 0.1 to 10 ng of unlabeled I. The 4, 5, 6, 7 and 8-hydroxy derivs. of I, known metabolites in man, showed less than 1.There are some commonly used reagents with their cas registry numbers 62180-98-7 and 867-44-7 in this article.5% cross-reaction with the antiserum. The specificity of the RIA was established when good agreement was obtained for the levels of I in patients' plasma using the RIA and a specific gas chromatog.-mass spectrometry method. The simplicity of the RIA makes this method attractive for the routine clin. monitoring of the plasma levels and/or exptl. protocols in which high sample throughput is required. .

Molecular Patterning of a Guanidinium/Orotate Mixed Monolayer through Molecular Recognition with Flavin Adenine Dinucleotide

Molecular Patterning of a Guanidinium/Orotate Mixed Monolayer through Molecular Recognition with Flavin Adenine Dinucleotide. Oishi, Yushi; Torii, Yuzuru; Kato, Takayuki; Kuramori, Miyuki; Suehiro, Kazuaki; Ariga, Katsuhiko; Taguchi, Kazuhiro; Kamino, Ayumi; Koyano, Hiroshi; Kunitake, Toyoki (Department of Applied Chemistry Faculty of Science and Engineering, Saga University, Saga 840, Japan). Langmuir, 13(3), 519-524 (English) 1997 American Chemical Society. CODEN: LANGD5. ISSN: 0743-7463. DOCUMENT TYPE: Journal CA Section: 33 (Carbohydrates) Section cross-reference(s): 22, 46 FAD undergoes multi-site mol. recognition at the air-water interface with mixed monolayers that are capable of complementary hydrogen bonding. Mol. images were obtained with an at. force microscope (AFM) for mixed monolayers of guanidinium (G) and orotate (O) amphiphiles transferred from pure water and from aq. FAD. The AFM image of the G/O mixed monolayer on FAD showed a periodic oblique pattern composed of two kinds of Me peaks with different heights, whereas that on pure water showed a periodic hexagonal pattern of only one kind of Me peak. 112-92-5 and 867-44-7 which are cas registry numbers of substances are two of reagents here. The two-dimensional mol. patterning as height difference is conceivably caused by rearrangement of monolayer components based on specific recognition by the FAD template mol. .