Detail of "87084-52-4"

Reference

Genetic identification of functions of TR-DNA transcripts in octopine crown galls

Genetic identification of functions of TR-DNA transcripts in octopine crown galls. Salomon, F.; Deblaere, R.; Leemans, J.; Hernalsteens, J. P.; Van Montagu, M.; Schell, J. (Lab. Genet. Virol., Vrije Univ. Brussel, St.-Genesius-Rode B-1640, Belg.). EMBO J., 3(1), 141-6 (English) 1984. CODEN: EMJODG. ISSN: 0261-4189. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 11 Seventeen in vivo and in vitro insertion mutations in the TR-region of the octopine Ti plasmid B6S3 of Agrobacterium tumefaciens were constructed. They include mutations in each of the 5 TR transcripts. All mutants retained their oncogenic properties and induced tumors with wild-type morphol. Detn. of opines in tumors induced by mutant Agrobacterium strains showed that prodn. of mannopine [87084-52-4] and agropine [70699-77-3] are TR-linked traits. Two transcripts are necessary for the synthesis of mannopine and a 3rd one for the conversion of mannopine into agropine. None of the mutations prevents the excretion of opines. In addn., none of the 5 transcripts is essential for transfer and integration of the TR-region.

Hairy root transformation in alfalfa (Medicago sativa L

Hairy root transformation in alfalfa (Medicago sativa L.). Spano, L.; Mariotti, D.; Pezzotti, M.; Damiani, F.; Arcioni, S. (Dip. Genet. Biol.Some commonly used reagents like 70699-77-3 and 87084-52-4 are used in this experiment. Mol., Univ. Roma "La Sapienza", Rome I-00185, Italy). Theor. Appl. Genet., 73(4), 523-30 (English) 1987. CODEN: THAGA6. ISSN: 0040-5752. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 11 The widely cultivated forage legume alfalfa (M. sativa L.) was transformed with the agropine type Agrobacterium rhizogenes NCPPB 1855. Sterile root and callus cultures were derived from tumorous hairy roots which were easily obtained independent of the plant variety or genotype. Plant regeneration, via somatic embryogenesis, was achieved only when a selected alfalfa line, characterized by high regenerative capability, was utilized. Genetic transformation was confirmed by the presence of agropine and T-DNA. Phenotypic alterations, mainly affecting the root system, were obsd. in transformed plants. The possibility that T-DNA-induced variations could be useful in the improvement of M. sativa is discussed. .