Detail of "886-86-2"

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Use of respiratory-cardiovascular responses of rainbow trout (Salmo gairdneri) in identifying acute toxicity syndromes in fish: Part 1

Use of respiratory-cardiovascular responses of rainbow trout (Salmo gairdneri) in identifying acute toxicity syndromes in fish: Part 1. Pentachlorophenol, 2,4-dinitrophenol, tricaine methanesulfonate and 1-octanol. McKim, James M.; Schmieder, Patricia K.; Carlson, Richard W.In this study， 111-87-5 and 87-86-5 are also used.; Hunt, Evelyn P.; Niemi, Gerald J. (Environ. Res. Lab., U. S. Environ. Protect. Agency, Duluth, MN 55804, USA). Environ. Toxicol. Chem., 6(4), 295-312 (English) 1987. CODEN: ETOCDK. ISSN: 0730-7268. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) An in vivo fish model was adapted to monitor respiratory-cardiovascular responses of spinally transected rainbow trout exposed to acutely toxic aq. concns. of 2 uncouplers of oxidative phosphorylation, PCP (I) [87-86-5] and 2,4-dinitrophenol (DNP) [51-28-5], and 2 narcotics, MS-222 [886-86-2] and 1-octanol [111-87-5]. The most evident toxic response to the uncouplers was a rapid 150-200% increase in ventilation vol. (VG) and O consumption (VO2) over the entire survival period. This caused an initial increase in total arterial O (TaO2) content of the blood, which then fell slowly as the tissues used more and more O to generate ATP. Arterial blood pressure and other blood measurements did not change appreciably in response to PCP, yet DNP caused increases in hematocrit (Hct) and Hb and slight decreases in total arterial CO2 (TaCO2) and arterial pH (pHa). In contrast to the uncouplers, the response of the respiratory-cardiovascular system of trout to toxic levels of narcotics was a dramatic slowing of all respiratory-cardiovascular functions. Whereas VG and VO2 decreased 40-50% from predose levels, O utilization increased 20-30%. The ventilation rate declined initially and then increased slowly until death occurred, but remained within the control range. As respiration declined TaO2 significantly decreased, as did pHa. In response to hypoxia, Hct increased substantially, and Hb increased slightly only in the 1-octanol exposure. A rapid 40-50% drop in heart rate (reflex bradycardia) was also obsd. The final phase of toxicity caused by both uncouplers and narcotics appeared to eventually produce acute tissue hypoxia, with a generalized loss of respiratory-cardiovascular coordination, and finally respiratory paralysis. Individual principal component analyses of the cardiovascular-respiratory responses of fish exposed to the 1st 2 principal components explained 68-76% of the variation in the 18 parameters analyzed. A 2-dimensional diagram of the 1st 2 principal components illustrated this multivariate response and the increased variability in the responses of exposed fish as compared with control fish. The general sets of responses described for these 2 fish acute toxicity syndromes have provided the initial information necessary to group similar responses caused by other chems. into a fish uncoupler syndrome, a fish narcosis syndrome, or some new syndrome. .

Gas-liquid chromatographic determination of residues of methanesulfonate of m-aminobenzoic acid ethyl ester in fish

Gas-liquid chromatographic determination of residues of methanesulfonate of m-aminobenzoic acid ethyl ester in fish. Sills, Joe B.; Luhning, Charles W. (Southeast. Fish Control Lab., Fish Wildl. Serv., Warm Springs, Ga., USA). J. Assoc. Off. Anal. Chem., 60(4), 961-2 (English) 1977. CODEN: JANCA2. DOCUMENT TYPE: Journal CA Section: 17 (Foods) Section cross-reference(s): 1 In this gas chromatog. procedure for detg. residues of methanesulfonate of m-aminobenzoic acid Et ester (MS-222) [886-86-2] in fish muscle, homogenized tissue is extd. with distd. water, and proteins are removed by coagulation with Cl3CCO2H, centrifugation, and filtration. After careful pH admjustment of the filtrate, MS-222 is partitioned into C6H6-Et2O and measured by alkali flame ionization gas chromatog. Tissues with known addns. of 1-19 .mu.g MS-222/g were analyzed, with recoveries of 84-95%.