Detail of "89699-91-2"

Reference

New neurotoxin from the venom of the large hornet (Vespa orientalis)

New neurotoxin from the venom of the large hornet (Vespa orientalis).Some chemicals with cas registry numbers like 89699-91-2 are also used. Tuichibaev, M. U.; Tashmukhamedov, B. A.; Magazanik, L. G. (Inst. Biokhim., Tashkent, USSR). Uzb. Biol. Zh., (6), 3-4 (Russian) 1983. CODEN: UZBZAZ. ISSN: 0042-1685. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) A neurotoxin, designated as orientotoxin [89699-91-2], was isolated from V. orientalis by initial gel filtration of the venom on Sephadex G75. A highly toxic fraction III, which had a phospholipid-cleaving property, was further fractionated on cellulose column (CM-52) and then rechromatographed 2-3 times to isolate individual proteins. Orientotoxin was isolated from the 2nd-fraction (cellulose chromatog.), which had no phospholipid-cleaving property and the LD50 was 0.50 mg/kg. Fraction III, which had phospholipase activity, had an LD50 of 0.65 mg/kg. Orientotoxin, after further purifn., cleaved only 1-acyllysophospholipids. The max. enzymic activity of orientotoxin was obsd. at pH 7.5 in the presence of 1 mM Cu2+. Na deoxycholate and Triton X 100 totally inhibited the toxin activity. The toxin substantially inhibited nerve endplate potential. Orientotoxin is a presynaptic neurotoxin. .

Orientotoxin - novel presynaptically acting neurotoxin from venom of the giant hornet Vespa orientalis

Orientotoxin - novel presynaptically acting neurotoxin from venom of the giant hornet Vespa orientalis. Tuichibaev, M. U.; Tashmukhamedov, B. A.; Gotgil'f, I. G.; Magazanik, L. G. (Inst. Biochem., Tashkent, USSR). Bioorg. Khim., 10(3), 318-22 (Russian) 1984. CODEN: BIKHD7. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Section cross-reference(s): 6 Orientotoxin (I) [89699-91-2] of V. orientalis was isolated by initial gel filtration of the lyophilized venom on Sephadex G-75, followed by chromatog. of the toxic fraction III (high phospholipase activity) on CM-cellulose 52, equilibrated with pH 7.6 0.05 M Tris-HCl buffer, elution with linear gradient 0. 89699-91-2 which is the cas registry number of some chemical is mentioned.0-0.3 M NaCl, and desalting with Biogel R-10. The fraction II, with no phospholipase activity, gave I. I has a mol. wt. of 18,000 and lysophospholipase activity. I can block the induced and the spontaneous release of the neuromediator from the presynaptic nerve membrane. .