Detail of "89992-70-1"

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Synthesis of nucleoside phosphoramidite for rna oligonucleotide synthesis

All Rights Reserved. Synthesis of nucleoside phosphoramidite for rna oligonucleotide synthesis. Zhang, Biliang; Cui, Zhiyong (Peop. Rep. China ). Faming Zhuanli Shenqing Gongkai Shuomingshu CN 1900103 A 24 Jan 2007,22pp. (Chinese). (People's Republic of China). CODEN: CNXXEV. APPLICATION: CN 2010-35849 18 Jul 2005. DOCUMENT TYPE: Patent CA Section: 33 (Carbohydrates) The general formula of nucleoside phosphoramidite is shown in formula I. Nucleoside phosphoramidites I [R1 is N-protected adenine, guanine, cytosine, uracil or relative derivs.; R2 is 4,4'-dimethoxytrityl or other protective groups for hydroxyl; R3 is aminophosphate ester or other phosphate groups; R4 is H, Me or other groups; R5 is 2,2-dimethyl-2-(o-nitrophenyl)acetyl or 2,2-dimethyl-2-(substituted o-nitrophenyl)acetyl] are prepd. 89992-70-1 which is the cas registry number of one of substances is just one of reagents here. The invention protects 2'-hydroxyl of nucleoside with [2,2-dimethyl-2-(o-nitro-phenyl)acetyl Me ether] (DNPA), which has compatibility with general protective groups for 5'-hydroxyl. DNPA group can be removed under mild conditions, thus the integrality of RNA is completely ensured. .

A chemical 5'-phosphorylation of oligodeoxyribonucleotides

A chemical 5'-phosphorylation of oligodeoxyribonucleotides. Horn, Thomas; Urdea, Mickey S. (Chiron Res. Lab., Chiron Corp., Emeryville, CA 94608, USA). DNA, 5(5), 421-6 (English) 1986. CODEN: DNAADR. ISSN: 0198-0238. DOCUMENT TYPE: Journal CA Section: 33 (Carbohydrates) Section cross-reference(s): 6 To circumvent the use of T4 polynucleotide kinase and ATP for the 5'-phosphorylation of synthetic oligodeoxyribonucleotides after deprotection and purifn., a new chem. phosphorylation reagent that can be used on automated DNA synthesis instruments, was developed. The phosphite-derived compd., bis(b-cyanoethoxy)-N,N-diisopropylaminophosphine, is coupled to a solid-supported fragment and oxidized under the same conditions as nucleoside phosphoramidites. The resultant 5'-phosphate oligomer is then fully deprotected in std. NH4OH soln. at elevated temp. Reverse-phase, high-pressure liq. chromatog., silica thin-layer chromatog. 50-89-5 and 89992-70-1 which are cas registry numbers of chemicals are mentioned., and 31P-NMR spectra of chem. phosphorylated thymidine were compared with a variety of phosphorus derivs. of thymidine. All evidence suggested that the nucleoside was converted to the 5'-phosphate form quant. with the reagent. Moreover, an excellent enzymic ligation reaction was obtained with a chem. phosphorylated oligonucleotide. .