Reference

Effect of the harvest period and mechanical damage on various forms of peroxidase and catalase in carrot roots

Effect of the harvest period and mechanical damage on various forms of peroxidase and catalase in carrot roots. Volokitina, M.Several substances are used for example 9003-99-0 and 9001-05-2 which are their cas registry numbers. V.; Zaitseva, A. L.; Karchik, O. N.; Loginova, L. N.; Nikolaeva, M. A. (Vses. Nauchno-Issled. Inst. Zashch. Rast., USSR). Dokl. Vses. Akad. S-kh. Nauk im. V. I. Lenina, (12), 20-1 (Russian) 1983. CODEN: DVASAW. ISSN: 0042-9244. DOCUMENT TYPE: Journal CA Section: 11 (Plant Biochemistry) The catalytic activity of oxidoreductases and their intracellular distribution were strongly related to the root ripeness, as detd. by harvesting time. Mech. damage affected most young tissues, changing the intracellular localization of both title enzymes. The peroxidase activity increased as a result of mech. damage more in young than old roots, and its activity in homogenate and the activity of membrane-bound enzyme were higher in young than old roots. The catalase activity of all enzyme forms was also higher in young (1st harvest time) than old (2nd harvest time) roots. Thus, a delayed harvest results in a decreased damage of the cell structure after mech. root traumas. .

Does glycolate decarboxylation occur in intact leaf peroxisomes?

Does glycolate decarboxylation occur in intact leaf peroxisomes?. Schmitt, Mark R.Some chemicals with cas registry numbers like 9001-05-2 and 79-14-1 are also used.; Chestnut, Matthew H.; Edwards, Gerald E. (Dep. Bot., Washington State Univ., Pullman, WA 99164-4230, USA). Plant Sci. Lett., 33(1), 115-23 (English) 1984. CODEN: PTSLAF. ISSN: 0304-4211. DOCUMENT TYPE: Journal CA Section: 11 (Plant Biochemistry) Release of 14CO2 during metab. of [1-14C]glycolate was studied in purified, intact and Triton X-100-solublized peroxisomes isolated from leaves of Secale cereale. Decarboxylation, apparently resulting from H2O2 attack on glyoxylate, was stimulated in both intact and solubilized peroxisomal prepns. by the catalase inhibitors aminotriazole and NaN3. CO2 evolution was also obsd. in solubilized peroxisomes in the absence of inhibitor when an amino donor was not provided for conversion of glyoxylate to glycine. Loss of CO2 from labeled glycolate in the absence of amino donors was reduced by addn. of exogenous catalase to the reaction medium. Intact peroxisomes showed no glycolate decarboxylation, whether or not amino donors were supplied. It appears that the CO2 release from glycolate obsd. in previous investigations may be an artifact of the peroxisomal prepns. and the assay systems used, and not a significant factor in photorespiratory metab. in vivo. .