Reference

The luteotropic effect of homoplastic pituitary pars distalis homogenate, PMSG, and HCG on the corpora lutea of the hypophysectomized frog, Rana cyanophlyctis (SCHN)

The luteotropic effect of homoplastic pituitary pars distalis homogenate, PMSG, and HCG on the corpora lutea of the hypophysectomized frog, Rana cyanophlyctis (SCHN). Pancharatna, M.; Saidapur, S. K. (Dep. Zool., Karnatak Univ., Dharwad 580 003, India). Gen. Comp. Endocrinol., 53(3), 375-80 (English) 1984. CODEN: GCENA5. ISSN: 0016-6480. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) Section cross-reference(s): 2 The effect of homoplastic pituitary pars distalis homogenate (PDH), pregnant mare serum gonadotropin (PMSG), and human chorionic gonadotropin (HCG) on the postovulatory follicles/corpora lutea (CL) of the frog R. cyanophlyctis was studied to elucidate the factors regulating the life span of the luteal cells. Ovulation and spawning was induced in hypophysectomized frogs using PDH. Starting from Day 1 of spawning 1/2 PDH, 50 IU PMSG, or 50 IU HCG was injected daily for 3 days. In the saline-injected control frogs, the granulosa lutein cells regressed markedly on Day 2 with a steady progressive increase in the pycnosis of their nuclei. The sudanophilic lipid droplets of the luteal cells were fine on Day 1 but became coarser and reduced in no. on subsequent days. Histochem., the luteal cell 3 b-homoserine dehydrogenase (3b-HSDH) and glucose 6-phosphate dehydrogenase (G-6-PDH) also decrease drastically by Day 2. 9028-13-1 and 9002-61-3 are also in the experiment. In PDH-treated frogs the granulosa lutein cells were healthy on all 4 days of the expt. The nuclear diam. of the luteal cells increased progressively due to PDH. The pycnosis of the luteal cells was limited to 7.6% on Day 4 due to PDH as opposed to 68% in the controls. Histochem., 3b-HSDH and G-6-PDH activities remained much higher than in the controls with abundant sudanophilic lipids (both fine and coarse) in the luteal cells of PDH treated frogs even on Day 4. PMSG treatment also maintained the granulosa lutein cells beyond their normal life span but the luteotropic effect was less than that of PDH. HCG was least effective. Evidently the structural integrity of CL in the frog can be extended beyond the normal life span by injecting PDH or PMSG. .

Monoclonal antibodies to human chorionic gonadotropin and its beta subunit

Monoclonal antibodies to human chorionic gonadotropin and its beta subunit. Furuhashi, Yoshihito; Mano, Hisao; Hattori, Senei; Goto, Setsuko; Tomoda, Yutaka (Sch. Med., Nagoya Univ., Nagoya, Japan). Nippon Sanka Fujinka Gakkai Zasshi, 35(12), 2415-20 (English) 1983. CODEN: NISFAY. ISSN: 0300-9165. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Using the technique of somatic cell fusion, monoclonal antibodies to human chorionic gonadotropin (hCG) and its b-subunit were produced. Three double-cloned hybridomas (named 4H10, 4G2, and 1D12) were obtained. Anti-hCG 4H10 antibody reacted with hCG, the a-subunit of hCG (a-hCG) and human LH (hLH); and anti-hCG 4G2 antibody reacted with hCG and the b-subunit of hCG (b-hCG). 9002-67-9 and 9002-61-3 which are cas registry numbers of substances are two of reagents here. Anti-b-hCG 1D12 antibody had the capacity to bind with free b-hCG but not with intact hCG, which suggests that the 1D12 antibody can recognize a determinant site that is unique to b-hCG. This unique epitope in b-hCG might be expressed in or near a part which is hidden in the intact hCG mol. .