Detail of "9025-65-4"

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Use of sodium trichloroacetate and mung bean nuclease to increase sensitivity and precision during transcript mapping

Use of sodium trichloroacetate and mung bean nuclease to increase sensitivity and precision during transcript mapping. Murray, Michael G. (Agrigenet. Adv. Sci. Co., Madison, WI 53716, USA). Anal. Biochem., 158(1), 165-70 (English) 1986. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 9 An improved method for mapping RNA transcript boundaries by the nuclease protection technique was developed. This method exploited the large (>20°) difference in the thermal stability of RNA-DNA and DNA-DNA duplexes in concd. chaotropic salt solns. At 45° in 3.0M Na trichloroacetate, RNA-DNA hybridization was very efficient, but DNA-DNA duplexes remained completely denatured. For many applications, this solvent system can eliminate the need to prep. probes that are free of competing or irrelevant DNA mols. Fifty- to 100-fold more RNA-DNA hybridization was obsd. when reassocn. is performed in 3.0M Na trichloroacetate than in solns. contg. high concns. of formamide. A comparison of the use of S1 nuclease or mung bean nuclease [9025-65-4]indicated that mung bean nuclease could produce more precise and less ambiguous nuclease protection patterns.

Antitumor effect of Serratia marcescens nuclease covalently bound to soluble dextran

Antitumor effect of Serratia marcescens nuclease covalently bound to soluble dextran. Kurinenko, B. M.; Belyaeva, M. I.; Cherepneva, I. E.; Viesture, Z. (Kazan State Univ., Kazan, USSR). Vopr. Onkol., 23(11), 94-8 (Russian) 1977. CODEN: VOONAW. ISSN: 0507-3758. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Serratia marcescens nuclease (E.C. 3.1.4.9) [9025-65-4] diazocoupled with m-aminobenzyloxymethyldextran [65879-82-5] (mol. wt. 20,000, 40,000, and 60,000 daltons) was 3-4 times as effective as the native enzyme as an inhibitor of Ehrlich ascites carcinoma in mice. The enzyme prepn. using dextran with a mol. wt. of 40,000 daltons produced the greatest neoplasm inhibition.