Detail of "90284-47-2"

Reference

Assay processes and materials therefor

Assay processes and materials therefor. Sidki, Ahmad Mohammed; Smith, David Stuart (Unilever PLC; Unilever N. V. , UK). Eur. Pat. Appl. EP 104926 A2 4 Apr 1984, 29 pp. DESIGNATED STATES: R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: G01N033-54. APPLICATION: EP 83-305735 26 Sep 1983. PRIORITY: GB 82-27536 27 Sep 1982. DOCUMENT TYPE: Patent CA Section: 1 (Pharmacology) Section cross-reference(s): 2, 15 Specific binding assays for immunoassays are described in which the labeled substance is linked to a luminescent substance (erythrosine [16423-68-0]) which shows delayed light emission after photo-excitation, with a lifetime >1 ms, (usually 10-13,000 ms). The material is measured in the presence of a substance able to prevent quenching by mol. O. 82169-60-6 and 56391-56-1 are also in the experiment. The assay has the advantage that the labeling substance can be chosen from a no. of stable substances, and the O-free conditions are not needed for the assay, since a quench-inhibiting substance (sulfite, bisulfite, or metabisulfite) is used. Thus, erythrosine-labeled primidone was prepd. by reacting 4-aminophenylprimidone [82169-60-6] with erythrosine-5-isothiocyanate [90284-47-2]. Antibodies were raised to the bovine serum albumin conjugate of diazotized 4-aminophenylprimidone. After purifn., these antibodies and the erythrosine-labeled primidone were used to assay primidone [125-33-7] stds. prepd. in normal human blood serum in incubation mixes which contain Na sulfite as the quench-inhibiting material. .