Detail of "9055-00-9"

Reference

Processing method for microbial cells and their effects on enzyme retention

Processing method for microbial cells and their effects on enzyme retention. Tsumura, Nobuzo; Kasumi, Takafumi; Ishikawa, Masako (Natl. Food Res. Inst., Tokyo, Japan). Shokuhin Sogo Kenkyusho Kenkyu Hokoku, 31, 71-5 (Japanese) 1976. CODEN: SSKKCY. DOCUMENT TYPE: Journal CA Section: 16 (Fermentations) Glucose isomerase [9055-00-9]-contg. Streptomyces was agglutinated by addn. of chitosan [9012-76-4] at pH 6. Lactobacillus was also agglutinated with chitosan. Agglutination was followed by acetylation or treatment with anionic high mol. wt. electrolytes. Some high mol. wt. anionic electrolytes that ppt. when mixed with chitosan and improve enzyme retention are polyacrylic acid [9003-01-4] and gum arabic. Drying or freezing the cells also enhanced enzyme retention.

Intrinsic kinetic constants of an immobilized glucose-isomerase

Intrinsic kinetic constants of an immobilized glucose-isomerase. Benaiges, M. D.; Sola, C.; De Mas, C. (Fac. Cienc., Univ. Auton. Barcelona, Bellaterra, Spain). J. Chem. Technol. Biotechnol., 36(10), 480-6 (English) 1986. CODEN: JCTBED. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Section cross-reference(s): 7 The isomerization of glucose [50-99-7] into fructose [57-48-7] using a com. immobilized glucose-isomerase [9055-00-9] has been studied and an initial transitory state of the enzyme, probably due to an establishment of stationary concn. profiles, was obsd. Operational conditions to minimize the effects of external and internal mass transfer were detd. using immobilized enzyme particles with diams. less than 0.064 mm. Thermal enzyme deactivation was insignificant if it was pre-treated with cobalt. The intrinsic kinetic consts. of the reversible-reaction Michaelis-Menten equation were calcd. in operational conditions free of mass transfer effects.