Detail of "9074-98-0"

Reference

Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis

Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. Amory, Antoine; Kunst, Frank; Aubert, Elisabeth; Klier, Andre; Rapoport, Georges (Dep. Biotechnol., Inst. Pasteur, Paris 75724, Fr.). J. Bacteriol., 169(1), 324-33 (English) 1987.Chemicals with cas numbers 9000-90-2 and 107120-72-9 also play role. CODEN: JOBAAY. ISSN: 0021-9193. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) The sacQ gene from B. licheniformis was cloned and expressed in B. subtilis. Deletion anal. shows that it encodes a 46-amino acid polypeptide homologous to the B. subtilis sacQ gene product. The polypeptide, when it is overexpressed, activates the expression of a no. of target genes in B. subtilis, all encoding secreted enzymes: alk. protease [9073-77-2], levansucrase [9030-17-5], b-glucanase [9074-98-0], xylanase [37278-89-0], and a-amylase [9000-90-2]. The max. stimulations measured for alk. protease and levansucrase were by a factor of 70 and 50, resp., when the sacQ gene from B. licheniformis was present on a multicopy plasmid in B. subtilis. The sacQ genes from B. subtilis and B. licheniformis, cloned in the same multicopy plasmid, were compared under the same conditions. The sacQ gene from B. licheniformis was more efficient than the sacQ gene from B. subtilis in producing the hypersecretion phenotype. The sacQ structural genes from B. subtlis and B. licheniformis were placed under the control of the same inducible promoter. Hypersecretion was specifically obtained under conditions of full induction of the promotrer. The target site of levansucrase regulation by sacQ was identified as a 440-base-pair fragment located in the 5'-noncoding region of sacB, suggesting transcriptional control. .

Stabilization of wines using an enzymic treatment

Stabilization of wines using an enzymic treatment. Datunashvili, E. N.; Ezhov, V. N.; Vorob'eva, E. V.; Ragimov, N. K.; Avizenis, V.; Aleknaviciute, R. (Vses. Nauchno-Issled. Inst. Vinograd. Vinodel. "Magarach", Yalta, USSR). Vinodel. Vinograd. SSSR, (7), 14-17 (Russian) 1976. CODEN: VIVSA6. DOCUMENT TYPE: Journal CA Section: 16 (Fermentations) Stabilization of table wines using pectofoetidin P10.times. and G10.times. (0.01-0.02%) followed by tannin and gelatin is suggested; gelatin clarification is combined with cold treatment and filtration is also carried out in the cold. Stabilization of fortified wines using the same pectolytic prepns. and treatment with .beta.-glucanase [9074-98-0] (30 units/g activity) immobilized on ECTEOLA-cellulose in a column at a flow rate of 600 L/h/kg carrier, followed by gelatin clarification combined with cold-aging and filtration is recommended.