Reference

Microbial oxidation of sterol side-chains

Microbial oxidation of sterol side-chains. Bhattacharyya, P. K.; Rao, M. Krishna; Natarajan, Rama Devi; Ramgopal, Malathi; Madyastha, Prema; Madyastha, K. M. (Dep.There are some reagents like 57-88-5 is used in this study. Org. Chem., Indian Inst. Sci., Bangalore 560 012, India). J. Indian Chem. Soc., 61(1), 1-15 (English) 1984. CODEN: JICSAH. ISSN: 0019-4522. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Several sterol-metabolizing microorganisms, Pseudomonas convexa, P. stutzeri, Micrococcus species, Cephalosporium longisporum, and Moraxella species were isolated from soil. All oxidized the 8-C side chain of cholesterol [57-88-5]. The Moraxella was the most versatile as it degraded cholesterol derivs. such as 3b-methoxycholest-5-ene [1174-92-1], 3b-chlorocholest-5-ene [910-31-6], 3,5-cyclocholestan-6-one [7657-63-8], and K cholesteryl sulfate [6614-96-6] to the corresponding 17-keto steroids in 10-50% yield in shake flasks. It also gave high yields of estrone [53-16-7] from both 19-hydroxy-3b-acetoxycholest-5-ene [750-59-4] and 19-hydroxy-3b-acetoxysitost-5-ene [20835-78-3]. Two new enzymes one carrying out the oxidative O-demethylation of 6b-methoxy-3,5-cyclocholestane [2867-93-8] and the other, the isomerization of isosteroids to normal 3b-hydroxy-D6-steroids were discovered. Immobilized Moraxella cells in agar carried out the side chain degrdn. of cholesteryl sulfate [1256-86-6] to yield 3b-hydroxyandrost-5-en-17-one sulfate [651-48-9] and the 19-hydroxy-3b-acetoxy derivs. of both cholesterol and sitosterol to estrone in high yields. The half life of entrapped cells was estd. to be 28 days. .

Stable emulsions and improved temperature monitoring films prepared from them

Stable emulsions and improved temperature monitoring films prepared from them. Koff, Arnold (Hoffmann-La Roche, Inc., USA). U.S. US 4045383 30 Aug 1977, 7 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: C09K003-34. NCL: 260008000. APPLICATION: US 69-885353 15 Dec 1969. DOCUMENT TYPE: Patent CA Section: 63 (Pharmaceuticals) Improved temp.-monitoring films, useful in thermog. and(or) thermometry, are prepd. from stable emulsions contg. 25-75% cholesteric liq. crystals, homogeneously distributed throughout the films. The films are protected and stabilized against aging and atm. by adding antioxidants to the film-forming emulsions. Thus, cholesteryl nonanoate [1182-66-7] 37.25, cholesteryl oleyl carbonate [17110-51-9] 12.75, cholesteryl chloride [910-31-6] 10.0, BHT [128-37-0] 0.50, and color 0.66 wt. parts were mixed together, heated to .apprx.120-5.degree. till a clear soln. was formed, and 60.66 wt. parts of this compn. were mixed with 400 wt. parts of a gelatin soln. (prepd. by mixing gelatin with sorbic acid and heating to 70-75.degree.) and homogenized. The resulting emulsion could be cast into a film.