Detail of "921-62-0"

Reference

Alterations in nicotinamide and adenine nucleotide systems during mixed-function oxidation of p-nitroanisole in perfused livers from normal and phenobarbital-treated rats

Alterations in nicotinamide and adenine nucleotide systems during mixed-function oxidation of p-nitroanisole in perfused livers from normal and phenobarbital-treated rats. Kauffman, Frederick C.; Evans, Roxanne K. (Sch. Med., Univ. Maryland, Baltimore, Md., USA). Biochem. J., 166(3), 583-92 (English) 1977. CODEN: BIJOAK. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Phenobarbital (I) [50-06-6] (1 mg/mL, orally) treatment of rats increased rates of p-nitroanisole (II) [100-17-4] O-demethylation in 0.2mM II-perfused livers approx. 5-fold. I treatment increased the NADP [53-59-8] to NADPH [53-57-6] ratio calcd. from liver concns. of 6-phosphogluconate [921-62-0] and ribulose 5-phosphate [4151-19-3], but not the NADP to NADPH ratio calcd. from malic enzyme (EC 1.1.1.38) [9080-52-8]; addn. of II produced a redn. of NADP calcd. from 6-phosphogluconate dehydrogenase (EC 1.1.1.44) [9073-95-4] activity. Both glucose 6-phosphate dehydrogenase (EC 1.1.1.49) [9001-40-5] and malic enzyme activities of liver were increased by I. Liver concns. of adenine nucleotides were not altered by I teatment, but the ATP [56-65-5] to ADP [58-64-0] ratio decreased during mixed-function oxidn. of II by livers of I-treated rats. Perfusion of liver with hexobarbital [50-09-9] (0.1mM) or aminopyrine (III) [58-15-1] (0.5mM) increased the NADP to NADPH ratio calcd. from malic enzyme; addn. of 2,4-dinitrophenol (25mM) to the perfusate contg. III decreased the ATP to ADP ratio and decreased the oxidn. of NADPH obsd. with III alone. Thus in the presence of an uncoupler, NADPH generation may exceed its utilization by mixed-function oxidn.

Effects of diets on concentrations of 6-phosphogluconate and fructose 2,6-bisphosphate in rat livers and an assay of fructose 2,6-bisphosphate with an improved method

Effects of diets on concentrations of 6-phosphogluconate and fructose 2,6-bisphosphate in rat livers and an assay of fructose 2,6-bisphosphate with an improved method. Sommercorn, James; Freedland, Richard A. (Sch. Vet. Med., Univ. California, Davis, CA 95616, USA). J. Nutr., 114(8), 1462-9 (English) 1984. CODEN: JONUAI. ISSN: 0022-3166. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) The effects were studied of diets that have different lipogenic potentials on hepatic concns. of 6-phosphogluconate [921-62-0] and fructose 2,6-diphosphate (I) [79082-92-1], both of which activate hepatic phosphofructokinase [9001-80-3]. Diets high in carbohydrate increased concns. of both effectors compared to a high protein (gluconeogenic) diet. The concn. of 6-phosphogluconate was assocd. with the lipogenic nature of the diet, and the range of its concn. matched that over which phosphofructokinase responds to 6-phosphogluconate in vitro. In contrast, the concn. of I was not assocd. with the lipogenic potential of the diets. I was either absent from liver or its concn. was 10-30-fold higher than the concn. that gives the maximal activation of phosphofructokinase in vitro. The results indicate that I and 6-phoshogluconate have different roles in the regulation of phosphofructokinase. I may be involved in switching hepatic carbohydrate metab. between gluconeogenesis and glycolysis, whereas changes in the concn. of 6-phosphogluconate may coordinate the disposition of glucose 6-phosphate between the oxidative branch of the hexosemonophosphate pathway and glycolysis. An enzymic assay for I was improved during these studies.