Reference

Immobilized oligopeptides

Immobilized oligopeptides. Cassani, Giovanni; Corti, Angelo; Riva, Ernesto; Soffientini, Adolfo; Parenti, Francesco (Gruppo Lepetit S.p.A. , Italy). Eur. Pat. Appl. EP 122969 A2 31 Oct 1984, 13 pp. DESIGNATED STATES: R: AT, BE, CH, DE, FR, GB, IT, LI, LU, NL, SE. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: C07C103-52; A61K047-00; B01D015-08; C07G011-00; G01N033-48. APPLICATION: EP 83-112555 14 Dec 1983. PRIORITY: GB 83-11136 25 Apr 1983. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Section cross-reference(s): 1 Oligopeptide ligands [e.g., D-alanyl-D-alanine [923-16-0] or poly(D-alanine) [26701-36-0] (3-15 units)] are linked directly or via spacer arms to water-insol. carriers (e.g., agarose [9012-36-6], dextran [9004-54-0], glass) to prep. adsorbents that can selectively adsorb antibiotics (e.g., teicoplanin [61036-62-2], vancomycin [1404-90-6], ristocetin A [11021-66-2]) that can bind to the terminal D-alanyl-D-alanine groups in growing cell walls of bacteria. The adsorbents are used to purify antibiotics from mixts., e.g., fermn. broth, to screen for antibiotics that can bind to the oligopeptide ligand on the adsorbent, and to det. antibiotics in body fluids. Thus, D-alanyl-D-alanine was coupled to activated CH-Sepharose 4B, and the adsorbent matrix thus produced was used to purify the antibiotic teicoplanin from soln. by selective adsorption and elution and to det. teicoplanin in urine by selective adsorption and elution followed by HPLC.

The triangle-relationship of b-lactam antibiotics

The triangle-relationship of b-lactam antibiotics. Liu, Jingjiang; Tang, Jiwei; Zhu, Minhui; Fan, Xiaoling (Dep. Chem., Nankai Univ., Tianjin 300071, Peop. 140224-86-8 and 923-16-0 are cas registry numbers. These chemicals are also mentioned in this article. Rep. China). Chin. Chem. Lett., 2(8), 653-4 (English) 1991. CODEN: CCLEE7. DOCUMENT TYPE: Journal CA Section: 26 (Biomolecules and Their Synthetic Analogs) Section cross-reference(s): 22, 34 The geometric parameters for D-Ala-D-Ala are optimized by mol. mechanics, giving, the most stable and the two next most stable conformations. The interaction model with the target enzyme is confirmed. The proposed triangular relationship could be found in different classes of b-lactams also. .