Detail of "92748-40-8"

Reference

Substrate properties of 5-fluorouridine diphospho sugars detected in hepatoma cells

Substrate properties of 5-fluorouridine diphospho sugars detected in hepatoma cells. Weckbecker, Gisbert; Keppler, Dietrich O. R. (Biochem. Inst., Univ. Freiburg, Freiburg/Br. D-7800, Fed. Rep. Ger.). Biochem.Except for chemicals metioned above, 92748-38-4 is also used. Pharmacol., 33(14), 2291-8 (English) 1984. CODEN: BCPCA6. ISSN: 0006-2952. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Nucleotide sugars derived from 5-fluorouridine [316-46-1] were studed in cultured AS-30D hepatoma cells as well as in kinetic enzyme assays in vitro in comparison with the physiol. uridine diphospho sugars. Hepatoma cells converted 5-fluoro [14C]uridine to 5-fluorouridine diphospho glucose (FUDP glucose) [79965-09-6] FUDP-galactose [92748-40-8], FUDP-N-acetylglucosamine [87810-49-9], FUDP-N-acetylgalactosamine [92934-61-7], and trace amts. of FUDP-glucuronate [92748-41-9], as analyzed by different systems of high-performance liq. chromatog. 5-Fluoro[14C]uridine and 14C-labeled uridine [58-96-8], at concns. of 5 mM in the culture medium, were phosphorylated by the cells during 60 min to similar amts. of 5-fluorouridine 3'-triphosphate [92748-37-3] and UTP [63-39-8], resp., while the synthesis of [14]FUDP-sugars was reduced to 14% as compared to that of [14C]UDP-sugars. FUDP-sugars, synthesized by chem. and enzymic procedures, were assayed in vitro as substrates for enzymes of UDP-sugar metab. Km And V values in a range comparable to that of the resp. UDP-sugars were detd. for FUDP-sugars in the reactions catalyzed by UDP-glucose pyrophosphorylase [9026-22-6], galactose-1-phosphate uridylyltransferase [9016-11-9], UDP-glucose 4-epimerase [9032-89-7], UDP-N-acetylglucosamine 2-epimerase [9037-71-2], glycogen synthase [9014-56-6], and UDP-glucose dehydrogenase [9028-26-6]. Expts. in hepatoma cells and with enzymes in vitro have revealed addnl. reactions of FUDP-sugar metab. demonstrating a metabolite pattern analogous to that of UDP-sugars. The amts. of FUDP-sugars formed relative to UDP-sugars in intact cells were smaller than suggested on the basis of their kinetic comparison in vitro. .