Detail of "93-11-8"

Reference

Specific 3A,3C,3E-0-trisulfonylation of b-cyclodextrin

Specific 3A,3C,3E-0-trisulfonylation of b-cyclodextrin. Fujita, Kahee; Tahara, Tsutomu; Yamamura, Hatsuo; Koga, Toshitaka (Fac. Pharm. Sci., Nagasaki Univ., Nagasaki 852, Japan). Denpun Kagaku, 38(2), 205-9 (Japanese) 1991. CODEN: DPNKAV. ISSN: 0021-5406. DOCUMENT TYPE: Journal CA Section: 33 (Carbohydrates) 3A,3C,3E-tri-O-(b-naphthylsulfonyl)-b-cyclodextrin (I) was regioselectively prepd. by the reaction of b-cyclodextrin with b-naphthylsulfonyl chloride in an alk. soln. Effect of the buffer concn. (Na2HPO4.12H2O mol/L) on the yield of I and on the reaction time required for the pH for the reaction mixt. to change from 12 to 8 was illustrated. The structure detn. of I was carried out by use of a regiochem. relationship between a 3-O-sulfonylcyclodextrin with a 6-O-sulfonylcyclodextrin through a cyclodextrin 2,3-alloepoxide and a 3,6-anhydrocyclodextrin.Except for chemicals metioned above, 93-11-8 and 101030-90-4 are also used. .

Derivatization of primary amines by 2-naphthalenesulfonyl chloride for high-performance liquid chromatographic assay of neomycin sulfate

Derivatization of primary amines by 2-naphthalenesulfonyl chloride for high-performance liquid chromatographic assay of neomycin sulfate. Tsuji, Kiyoshi; Jenkins, Kevin M. (Control Res. Dev., Upjohn Co., Kalamazoo, MI 49001, USA). J. Chromatogr., 369(1), 105-15 (English) 1986. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) A normal phase HPLC method was developed for the assay of neomycin [1404-04-2]. The method involves precolumn derivatization with 2-naphthalenesulfonyl chloride (I) [93-11-8] followed by extn. in CHCl3 and chromatog. using a normal phase silica column with detection at 254 nm. The std. curve for the HPLC assay of neomycin is linear with a correlation coeff. of 0.9996 over the range of 0.02 to 0.4 mg/mL. Neomycin B [119-04-0], and C [66-86-4], and neamine [3947-65-7] can be sepd. and quantified isocratically with relative std. deviations of 0.92% and 1.4% for neomycin (B + 1/2C) and neamine, resp. Prednisolone is used as an internal std. to aid in quantification. Mass spectrometric data confirms that neomycin is derivatized at all the six primary amines on the neomycin mol. Eight lots of neomycin were used to compare the HPLC [I and 1-fluoro-2,4-dinitrobenzene (DNFB) [70-34-8]], gas-liq. chromatog. and microbiol. assay methods. The av. results of the I-HPLC method fell between those of the microbiol. and DNFB-HPLC methods. Also, good correlation of the neomycin C contents in neomycin were obtained between the I-HPLC and DNFB-HPLC methods.