Detail of "93229-61-9"

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Method for transferring insect-resistance gene into Elymus sibiricus (Chuancao no

All Rights Reserved. Method for transferring insect-resistance gene into Elymus sibiricus (Chuancao no.2). Yang, Zhirong; Li, Daxu; Zhang, Jie; Zhao, Jian (Sichuan University, Peop. Rep. China). Faming Zhuanli Shenqing Gongkai Shuomingshu CN 1888071 A 3 Jan 2007, 16pp. (Chinese). (People's Republic of China). CODEN: CNXXEV. APPLICATION: CN 2010-21175 27 Jun 2005. DOCUMENT TYPE: Patent CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 11 The title method comprises the steps of: (1) prepg. an explant from the mature embryo of Elymus sibiricus (chuancao No.2), (2) culturing the explant in MS medium contg. 5.0mg/L 2,4-D and 0.05mg/L kinetin to induce callusogenesis, culturing the calluses for 8 wk, selecting yellowy granular embryo calluses, culturing for 4d in a medium (pH 5.6) contg. 25% MS medium, 5.0mg/L 2,4-D, 100mM acetosyringone, 2% sucrose, 1% glucose and 1% agar powder, and invading with Agrobacterium transformed by an expression vector contg. target gene, (3) screening resistant calluses in a selective medium contg. 40mg/L hygromycin, and removing Agrobacterium, (4) inducing in a medium contg. 1.5mg/L 2,4-D, 0.1mg/L kinetin and 40mg/L hygromycin to obtain somatic cell embryos, (5) sprouting, and screening by 40mg/L hygromycin, and (6) detecting by mol. 525-79-1 and 93229-61-9 which are cas registry numbers of substances are two of reagents here. identification methods and insect-resistance tests to obtain transgenic insect- resistant Elymus sibiricus (Chuancao No.2). The transgenic Elymus sibiricus (chuancao No.2) has locust resistance, and can be expected to use for accelerating animal husbandry development and preventing grasslands from desertification. .

Rapid identification of non tuberculous mycobacteria by restriction pattern analysis

All Rights Reserved. Rapid identification of non tuberculous mycobacteria by restriction pattern analysis. Araya R, Pamela; Velasco R, Maritza; Fernandez O, Jorge (Unidad de Desarrollo, Instituto de Salud Publica de Chile, Santiago de Chile, Chile). Revista Medica de Chile, 134(7), 868-873 (Spanish) 2006 Sociedad Medica de Santiago. CODEN: RMCHAW. ISSN: 0034-9887. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 10 Background: The frequency of diseases caused by non tuberculous mycobacteria has increased in the last years. Their clin. diagnosis is difficult, mainly in immunocompromised patients. The identification of these mycobacteria by traditional methods is based on phenotypic characteristics and the results are obtained two to four weeks after their isolation in primary cultures. Aim: To report a new identification method for non tuberculous mycobacteria. Material and methods: The restriction pattern anal. method was implemented. 93229-61-9 and 81295-18-3 which are cas registry numbers are also used here. It is based on the amplification, using polymerase chain reaction (PCR), of a polymorphic region of 440 base pairs that codifies Hsp65 protein, followed by a digestion with BstE II and Hae III restriction enzymes. The results were compared with patterns established for each strain. Results: Sixty four strains of mycobacteria obtained from clin. samples and seven ref. mycobacteria, were identified using the traditional methods and restriction pattern anal. The latter method identified the same strain as the former in 87.5% of cases. In the remainder 12.5% of cases there was no agreement between both methods. In these, the sequencing of a fragment of a gene that codifies 16S rRNA, confirmed the correct identification by restriction patterns. Conclusions: Restriction pattern anal. is a rapid identification method for non tuberculous mycobacterial strains. .