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Effect of gastrin-releasing peptide on sperm functions

Effect of gastrin-releasing peptide on sperm functions. Levy, Rachel; Eustache, Florence; Pilikian, Sylvia; Clavel, Chantal; Cordonier, Helene; Benchaib, Mehdi; Lornage, Jacqueline; Pinatel, Marie-Claude; Guerin, Jean Francois (Laboratoire de Biologie de la Reproduction et du Developpement, Hopital Edouard Herriot, Lyon 69437, Fr.). Molecular Human Reproduction, 2(11), 867-872 (English) 1996 Oxford University Press. CODEN: MHREFD. ISSN: 1360-9947. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Male infertility can be related to defects in motility, capacitation, acrosome reaction, binding and penetration of the zona pellucida. While different in-vitro techniques (such as micromanipulation which is complicated and expensive) are available for the treatment of male infertility, several pharmacol. agents have been shown to increase fertilizing capacity under accurate exptl. conditions. Gastrin-releasing peptide (GRP, the mammalian homolog of the amphibian skin peptide bombesin) is present in the reproductive tract and expressed by the pregnant ovine endometrium prior to attachment and throughout the pregnancy. A bombesin-like peptide resulting from alternate splicing of the GRP gene in testis has been detected in primates. In this study, we have tested the ability of GRP to enhance human sperm functions such as motility, capacitation, zona binding and acrosome reaction. Anal. of sperm motility was performed with the ATS 20 computer-assisted semen anal. (CASA) system. Zona binding was analyzed using intact human unfertilized oocytes and selective labeling of spermatozoa with two fluorochromes. Our results did not show any pos. effect of GRP on these parameters under our exptl. 93755-85-2 which is the cas registry number of one of substances is just one of reagents here. conditions. However, when GRP at the concn. of 100 nM was added after ionophore treatment, the percentage of reacted cells increased significantly compared with situations where each agent was used alone. This led us to suppose that the role of bombesin in the different stages of fertilization might not exclude other unknown factors. .