Detail of "94991-73-8"

Reference

High-performance liquid chromatographic assay for mexiletine enantiomers in human plasma

High-performance liquid chromatographic assay for mexiletine enantiomers in human plasma. Grech-Belanger, Odette; Turgeon, Jacques; Gilbert, Marcel (Sch. Pharm., Laval Univ., Ste-Foy, PQ G1K 7P4, Can.). J. Chromatogr., 337(1), 172-7 (English) 1985. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The simultaneous detn. of mexiletine enantiomers in human blood involved derivatization of each isomer with 2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl isothiocyanate (GITC) followed by HPLC sepn. using UV absorption. Blood samples contg. the enantiomers were extd. with Et2O, the exts. were evapd. to dryness, the residue reconstituted with GITC in DMF (DMF) and vortexed, and hydrazine in DMF was added to the resulting mixt. Chromatog. sepn. was performed on a Waters C18 mBondpak column (30 cm ′ 4 mm I.D., 10 mm particle size) using a mobile phase which consisted of MeOH-10 mM phosphate buffer, pH 5.5 () at a flow-rate of 2 mL/min. Calibration curves were linear over a concn. range of 51.9-389.5 ng/mL of each enantiomer. The percentage coeff. of variation for inter- and intra-day anal. was < 5% except for the inter-day variations at the lowest concn. studied (20.7 ng/mL) which were 10.3 and 10.5% for S(+)- [94991-72-7] and R(-)-mexiletine [94991-73-8], resp. This method was used for the detn. of these enantiomers in plasma of a volunteers to whom racemic mexiletine (I) [61079-93-4] had been administered orally.

High-performance liquid chromatographic determination of mexiletine enantiomers in plasma using direct and indirect enantioselective separations

High-performance liquid chromatographic determination of mexiletine enantiomers in plasma using direct and indirect enantioselective separations. Lanchote, Vera Lucia; Bonato, Pierina Sueli; Dreossi, Sonia A. C.; Goncalves, Paulo V. B.; Cesarino, Evandro J.Chemicals with cas numbers 94991-73-8 and 94991-72-7 also play role.; Bertucci, Carlo ( Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil). Journal of Chromatography, B: Biomedical Applications, 685(2), 281-289 (English) 1996 Elsevier. CODEN: JCBBEP. ISSN: 0378-4347. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Two methods were developed for the detn. of mexiletine enantiomers in plasma samples suitable for studies on the stereoselective disposition of this drug. Both methods used fluorescence detection to improve sensitivity and selectivity. The direct enantioselective sepn. was based on the chiral resoln. of mexiletine-2-naphthamide derivs. on a Chiralcel OJ column. The calibration curves were linear over the concn. range 50-500 ng/mL for each enantiomer; therefore the method can be used only for therapeutic monitoring, drug interaction and multiple dose pharmacokinetic studies. The indirect method was based on the formation of diastereomers using o-phthaldialdehyde and N-acetyl-L-cysteine reagents. The diastereomers were resolved on a reversed-phase RP-18 column. The method proved to be suitable for single or multiple dose pharmacokinetic studies based on the low quantification limit (1 ng/mL) and the broader linear range (1-1000 ng/mL) obtained. .