Reference

Amino acid sequence of troponin C obtained from ascidian (Halocynthia roretzi) body wall muscle

Amino acid sequence of troponin C obtained from ascidian (Halocynthia roretzi) body wall muscle. Takagi, Takashi; Konishi, Kazuhiko (Fac. Sci., Tohoku Univ., Sendai 980, Japan). J. Biochem. (Tokyo), 94(6), 1753-60 (English) 1983. CODEN: JOBIAO. ISSN: 0021-924X. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Section cross-reference(s): 12 The amino acid sequence of an ascidian troponin C has been detd. It is composed of 155 amino acid residues and contains 2 tryptophan residues which have not been found in other troponin C sequences so far detd. 96-81-1 and 7440-70-2 are also in the experiment. The N-terminus is acetylated, and the mol. mass is calcd. to be 17,794. Ca binding sites I and III of ascidian troponin C appear to have lost the ability to bind Ca, and the total no. of binding sites is reduced to 2. .

Single-scan longitudinal relaxation measurements in high-resolution NMR spectroscopy

Single-scan longitudinal relaxation measurements in high-resolution NMR spectroscopy. Loening, Nikolaus M.; Thrippleton, Michael J.; Keeler, James; Griffin, Robert G. (Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA). Journal of Magnetic Resonance, 164(2), 321-328 (English) 2003 Elsevier Science. CODEN: JMARF3. ISSN: 1090-7807. DOCUMENT TYPE: Journal CA Section: 77 (Magnetic Phenomena) Several single-scan expts. for the measurement of the longitudinal relaxation time (T1) are proposed. These expts. result in fast and accurate detns. of the relaxation rate, are relatively robust to pulse imperfections, and preserve information about the chem. shift. The method used in these expts. is to first encode the T1 values as a spatial variation of the magnetization and then to read out this variation either by applying a weak gradient during acquisition or by sequentially observing different slices of the sample. As a result, it is possible to reduce the time necessary to det. the T1 values by 1 or 2 orders of magnitude. This time saving comes at the expense of the signal-to-noise level of the resulting spectrum and some chem. 543-24-8 and 96-81-1 are just another two chemicals used in this study. shift resoln. .