Reference

The effect of the antiprogestins RU 486 and ZK 98734 on the morphology of human decidual cells in culture

The effect of the antiprogestins RU 486 and ZK 98734 on the morphology of human decidual cells in culture. Dong, Liying; Zhou, Jian; Xue, Ying; Zheng, Shurong (1st Teach. Hosp., Beijing Med. Univ., Beijing 100034, Peop. Rep. China). Zhongguo Linchuang Yaoli Zazhi, 7(3), 169-73 (Chinese) 1991. CODEN: ZLYZE9. ISSN: 1001-6821. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The effects of RU 486 and ZK 98734 were studied on the morphol. of human decidual cells (DE). DE obtained from women in early pregnancy (45-50 days amenorrhea from the last menstrual period) were grown in tissue culture. Both RU 486 and ZK 98734 inhibited the proliferation and differentiation of DE. The morphol. changes of DE induced by the antiprogestins were dose- and time-dependent within the concn. range from 5 ′ 10-4 to 5 ′ 10-6 mol/L. Their inhibitory effect was reversible after removal of the drugs. The effect of RU 486 is slightly stronger than that of ZK 98734 at the same dosage (5 ′ 10-5 mol/L).Several substances with their cas registry numbers 84371-65-3 and 97747-88-1 may be metioned in this study. Thus, the development of human DE in culture may be affected directly by the 2 antiprogestins and their effect is reversible. .

Distribution of uteroglobin in the rabbit endometrium after treatment with an anti-progesterone (ZK 98

Distribution of uteroglobin in the rabbit endometrium after treatment with an anti-progesterone (ZK 98.734): an immunocytochemical study. Hegele-Hartung, Christa; Beier, Henning M. (Dep. Anat. Reprod. Biol., Univ. Aachen, Aachen D-5100, Fed. Rep. Ger.). Hum. Reprod., 1(8), 497-505 (English) 1986. CODEN: HUREEE. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The effect of the synthetic steroid ZK 98734 (I) [97747-88-1], an anti-progesterone [57-83-0] with high affinity for the progesterone receptor, on uteroglobin [9060-09-7] distribution in the rabbit endometrium was studied by means of immunocytochem. Rabbits were treated with I during the 2nd, 3rd, and 4th day of pseudopregnancy. At 5-8 days of pseudopregnancy, the uteri were processed for immunocytochem. by using the peroxidase-antiperoxidase (PAP) and protein A-gold techniques. Uteroglobin synthesis and release could be inhibited by the anti-progesterone treatment. On day 5 and 6, there was no labeling of the uterine secretions and only a few diffusely labeled nonciliated cells could be seen in the surface and glandular epithelium. The inhibition was reversible in so far as on day 7 and day 8 the rabbit endometrium exhibits a clear labeling of the uterine secretion as well as an increase in pos. reaction in the epithelial cells lining the glands. In all treated animals, the intracellular uteroglobin labeling was confined to the Golgi complex and secretory vesicles with a significant increase from the 5th to the 8th day of pseudopregnancy. Together with the described morphol.In this experiment, several chemicals are used like 57-83-0 and 97747-88-1 changes these results indicate that I is capable of inducing a delayed secretion in the rabbit endometrium, which is comparable to the delay in secretion caused by postcoital estradiol treatment. However, the antigestagen effect is probably due to a different mechanism of endocrine interference with preimplantation. The most exciting consequence, so far, is the prolongation of progesterone action after the antiprogesterone treatment had ended. .