Reference

The toxicological implications of the interaction of butylated hydroxytoluene with other antioxidants and phenolic chemicals

The toxicological implications of the interaction of butylated hydroxytoluene with other antioxidants and phenolic chemicals. Thompson, D. C.; Trush, M. A. (Sch. Hyg. Public Health, Johns Hopkins Univ., Baltimore, MD 21205, USA). Food Chem. Toxicol., 24(10-11), 1189-95 (English) 1986. CODEN: FCTOD7.Several reagents with their cas registry numbers 97-53-0 and 128-37-0 are used here. ISSN: 0278-6915. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Butylated hydroxyanisole (BHA) [25013-16-5] enhanced both the in vitro peroxidase-catalyzed covalent binding of butylated hydroxytoluene (BHT) [128-37-0] to microsomal protein and the formation of BHT-quinone methide [2607-52-5]. Eugenol [97-53-0], methylparaben [99-76-3], vanillin [121-33-5], guaiacol [90-05-1], ferulic acid [1135-24-6] and several other phenolic compds. commonly used in food and cosmetic products also enhanced the metabolic activation of BHT. BHA was the most effective compd. tested. Microsomes from lung, bladder, kidney medulla and small intestine of various animal species, including man, were also able to support this interaction of BHA and BHT using either hydrogen peroxide or arachidonic acid as the substrate. These in vitro observations were extended to an in vivo mouse lung model. S.c. injections of BHA significantly enhanced the lung/body wt. ratio of mice given i.p. injections of subthreshold doses of BHT. The toxicol. implications of the interactions of BHT with other antioxidants and phenolic chems. and their potential relevance to human risk are discussed. .

Simultaneous HPLC determination of Dowicil 200 and methyl- and propylparabens in aqueous-based mascaras and cosmetic powders by the internal standard method

Simultaneous HPLC determination of Dowicil 200 and methyl- and propylparabens in aqueous-based mascaras and cosmetic powders by the internal standard method. Buchanan, Jo Anne; Philp, Roy (Noxzema Inc., Toronto, ON M8Y 3H7, Can.). LC-GC, 5(1), 52-5 (English) 1987. CODEN: LCGCE7. ISSN: 0888-9090. DOCUMENT TYPE: Journal CA Section: 62 (Essential Oils and Cosmetics) Section cross-reference(s): 64 Levels of 0.02-0.30% Dowicil 200 (N-(3-chloroallyl)hexaminium chloride] [51229-78-8], a preservative, were detd. quant. by HPLC. In aq. soln., the preservative produced HCHO [50-00-0] which reacted with water to form methylene glycol [463-57-0]. Methylene glycol was condensed with 2,4-pentanedione [123-54-6] and NH3 to yield DDL [1079-95-4], an end product of lutidine that absorbs in the UV. Therefore, the Dowicil 200 could be assayed by monitoring the liberation of methylene glycol. Preservatives in cosmetics also may contain methyl- [99-76-3], propyl- [94-13-3], and/or butylparaben [94-26-8] in combination with Dowicil 200. With this method, Dowicil 200 and the various parabens can be extd. together and measured using a UV-vis detector at 254 nm.Except for chemicals metioned above, 94-26-8 and 50-00-0 are also used. .