Reference

Method of producing reduced coenzyme Q10

Method of producing reduced coenzyme Q10. Ueda, Takahiro; Kitamura, Shiro; Ueda, Yasuyoshi (Kaneka Corporation, Japan). PCT Int. Appl.In this study， 110-54-3 and 992-78-9 are also used. WO 2003006412 A1 23 Jan 2003, 21 pp. DESIGNATED STATES: W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NO, NZ, OM, PH, PL, PT, RO, RU, SD, SE, SG, SI, SK, SL, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VN, YU, ZA, ZM, ZW, AM, AZ, BY, KG, KZ, MD, RU, TJ, TM; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, CY, DE, DK, ES, FI, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT, SE, SN, TD, TG, TR. (Japanese). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: C07C043-23. ICS: C07C041-26. APPLICATION: WO 2002-JP7147 15 Jul 2002. PRIORITY: JP 2001-214482 13 Jul 2001; JP 2002-114877 17 Apr 2002. DOCUMENT TYPE: Patent CA Section: 25 (Benzene, Its Derivatives, and Condensed Benzenoid Compounds) Section cross-reference(s): 1, 17, 62, 63 Provided is a method of efficiently producing reduced coenzyme Q10 having excellent qualities which is useful in foods, foods with nutrient function claims, foods for specific health uses, nutritional supplements, tonics, animal drugs, drinks, feeds, cosmetics, drugs, remedies, preventives and so on. This method is suitable for industrial prodn. thereof. A method of producing reduced coenzyme Q10 comprises reducing oxidized coenzyme Q10 in an aq. medium with the use of a hydrosulfite wherein the redn. is carried out in the coexistence of a salt and/or under deoxygenated conditions at pH 7 or below. The formation of the oxidized coenzyme Q10 as a byproduct due to oxidization can be minimized, thereby giving reduced coenzyme Q10 having excellent qualities in a high yield. Thus, 100 g coenzyme Q10 (99.4% purity) was slowly added to a soln. of 80 g NaOCl (375%) in 1,100 g 10% aq. NaCl with stirring, allowed to react at 48° and pH 4-6 for 2 h, and treated with 1,000 g hexane, followed by removing the aq. layer, washing the heptane phase six-times with 1,000 g satd. aq. NaCl adjusted to pH to 3 with HCl, to give a hexane soln. of reduced coenzyme Q10 with a reduced coenzyme Q10/oxidized coenzyme Q10 wt. ratio of 99.5/0.5 (99% yield). .

Difference in antioxidant activity between reduced coenzyme Q9 and reduced coenzyme Q10 in the cell: studies with isolated rat and guinea pig hepatocytes treated with a water-soluble radical initiator

Difference in antioxidant activity between reduced coenzyme Q9 and reduced coenzyme Q10 in the cell: studies with isolated rat and guinea pig hepatocytes treated with a water-soluble radical initiator.Some chemicals with cas registry numbers like 992-78-9 and 5677-54-3 are also used. Matsura, Tatsuya; Yamada, Kazuo; Kawasaki, Takashi (Sch. Med., Hiroshima Univ., Hiroshima, Japan). Biochim. Biophys. Acta, 1123(3), 309-15 (English) 1992. CODEN: BBACAQ. ISSN: 0006-3002. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) A possible difference in antioxidant activity between reduced coenzyme Q9 (CoQ9H2) and reduced coenzyme Q10 (CoQ10H2) in animal cells was studied by incubation of hepatocytes with a hydrophilic radical initiator, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). Two kinds of hepatocytes differing in their content of CoQ homologs were used: rat, total (oxidized plus reduced) CoQ9: total CoQ10 6:1, guinea pig 1:5. The sum of total CoQ9 and CoQ10 in rat and guinea pig hepatocytes was about 780 and 400 pmol/mg protein, resp. The concn. of CoQ9H2 in rat hepatocytes decreased linearly after the addn. of AAPH, whereas that of oxidized CoQ9 showed a reciprocal increase. No loss of cell viability or increase of lipid peroxidn. was obsd. until most of the CoQ9H2 had been consumed. Cellular CoQ9H2 was consumed probably through scavenging of lipid peroxyl radicals produced by incubation with AAPH. On the other hand, CoQ10H2 was not significantly consumed in the AAPH-treated rat hepatocytes during incubation compared with the control cells. In guinea pig hepatocytes, cellular CoQ10H2 as well as CoQ9H2 was consumed by addn. of AAPH. a-Tocopherol also showed linear consumption with incubation time regardless of the cell types used. It is concluded that CoQ9H2, together with a-tocopherol, constantly acts as a potential antioxidant in hepatocytes when incubated with AAPH, whereas CoQ10H2 mainly exhibits its antioxidant activity in cells contg. CoQ10 as the predominant CoQ homolog. .