Detail of "99287-08-8"

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Direct inactivation of viruses by human granulocyte defensins

Direct inactivation of viruses by human granulocyte defensins. Daher, Kathleen A.; Selsted, Michael E.; Lehrer, Robert I. (Cent.In this study， 99287-07-7 and 99287-08-8 are also used. Health Sci., Univ. California Los Angeles, Los Angeles, CA 90024, USA). J. Virol., 60(3), 1068-74 (English) 1986. CODEN: JOVIAM. ISSN: 0022-538X. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Human neutrophils contain a family of microbicidal peptides known as defensins. One of these defensins, human neutrophil peptide (HNP)-1, was purified, and its ability to directly inactivate several viruses was extensively tested. Herpes simplex virus (HSV) types 1 and 2, cytomegalovirus, vesicular stomatitis virus, and influenza virus A/WSN were inactivated by incubation with HNP-1. Two nonenveloped viruses, echovirus type 11 and reovirus type 3, were resistant to inactivation. Purified homologous peptides HNP-2 and HNP-3 had HSV-1-neutralizing activities approx. equal to that of HNP-1. Inactivation of HSV-1 by HNP-1 depended on the time, temp., and pH of incubation. Antiviral activity was abrogated by low temp. or prior redn. and alkylation of the defensins. Addn. of serum or serum albumin to the incubation mixts. inhibited neutralization of HSV-1 by HNP-1. D. gradient sedimentation techniques were used to demonstrate that HNP-1 bound to HSV-1 in a temp.-dependent manner. Binding of defensin peptides to certain viruses may impair their ability to infect cells. .

The levels and biologic action of the human neutrophil granule peptide HP-1 in lung tumors

The levels and biologic action of the human neutrophil granule peptide HP-1 in lung tumors. Bateman, Andrew; Singh, Ava; Jothy, Serge; Fraser, Richard; Esch, Fred; Solomon, Samuel (Dep. Med., R. Victoria Hosp., Montreal, PQ H3A 1A1, Can.). Peptides (Fayetteville, N. Y.), 13(1), 133-9 (English) 1992. CODEN: PPTDD5. ISSN: 0196-9781. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) HP-1 is the most abundant human representative of a recently discovered class of neutrophil cystine- and arginine-rich peptides. These peptides have many potentially regulatory activities expressed at nanomolar concns. To establish the levels of HP-1 that can accumulate in human lung tumors and nondiseased lung fragments, tissues were extd. for their peptide content. The exts. were purified on reverse phase HPLC, and HP-1 and related peptides were identified by sequence anal. and their concns. in the tissue quantitated by amino acid anal. Immunohistochem. was performed and strongly suggests that HP-1 is confined to granulocytes under most circumstances, and indicates that the levels of HP-1 measured in the tumors reflect the levels obtained when solid tissue is infiltrated by neutrophils. The max. obsd. levels were 26 nmol per g wet wt. of tissue. Attempts were then made to correlate this level to the cytotoxic potential of HP-1 by performing in vitro cytotoxicity dose-response curves on several cell lines. Most cells were killed at between 1 and 8 mM, and the response depended on the growth conditions of the cells. 99287-08-8 is just another one chemical used in this study. The levels of HP-1 that accumulate in tumors can exceed the in vitro cytolytic concns. The levels are also considerably in excess of those required to exert in vitro regulatory actions. .