10560-45-9 Usage
General Description
2,3-DIPHENYL-5-(4-METHOXYPHENYL)TETRAZOLIUM CHLORIDE, also known as MTT, is a yellow tetrazolium dye used in cell viability and proliferation assays. It is commonly used to assess the metabolic activity of cells, as MTT is reduced by live cells to form insoluble formazan crystals, which can be dissolved and measured spectrophotometrically. MTT is commonly used in a variety of research fields, including cell biology, drug development, and toxicology, and is widely regarded as a reliable and sensitive indicator of cell viability. It is important to handle MTT with caution, as it is potentially hazardous if inhaled or ingested, and skin and eye contact should be avoided.
Check Digit Verification of cas no
The CAS Registry Mumber 10560-45-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,5,6 and 0 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 10560-45:
(7*1)+(6*0)+(5*5)+(4*6)+(3*0)+(2*4)+(1*5)=69
69 % 10 = 9
So 10560-45-9 is a valid CAS Registry Number.
InChI:InChI=1/C20H17N4O.ClH/c1-25-19-14-12-16(13-15-19)20-21-23(17-8-4-2-5-9-17)24(22-20)18-10-6-3-7-11-18;/h2-15H,1H3;1H/q+1;/p-1
10560-45-9Relevant articles and documents
1-Chlorobenzotriazole-Mediated Ring Closure of 1,3,5-Triarylformazans: Improved Syntheses of 2,3,5-Triaryl-2H-tetrazolium Salts
Katritzky, Alan R.,Belyakov, Sergey A.,Lam, Jamshed N.,Durst, H. Dupont,Karpenko, Dmitrii V.
, p. 73 - 80 (2007/10/02)
Oxidative ring closure of mono- and bis-triarylformazans mediated by easily available 1-chlorobenzotriazole leads to the corresponding 2,3,5-triaryl-2H-tetrazolium chlorides in yields of 70-97percent.
