114956-81-9 Usage
Uses
Different sources of media describe the Uses of 114956-81-9 differently. You can refer to the following data:
1. Laminin from human fibroblasts has been used: in coating six-well plates for wound healing assay as a substrate in cell adhesion and spreading assay for differentiation of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) toward dopaminergic neurons in corneal endothelial cell wound healing (migration) assay and corneal endothelial cell barrier assayLaminin from human fibroblasts is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
2. Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
General Description
Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.Biological activity: Tested for the promotion of adherence of HT-1080 cells.Species specificity: Active on most mammalian cells.
Biochem/physiol Actions
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.
Check Digit Verification of cas no
The CAS Registry Mumber 114956-81-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,4,9,5 and 6 respectively; the second part has 2 digits, 8 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 114956-81:
(8*1)+(7*1)+(6*4)+(5*9)+(4*5)+(3*6)+(2*8)+(1*1)=139
139 % 10 = 9
So 114956-81-9 is a valid CAS Registry Number.
