1230-27-9 Usage
Description
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is a chemical compound utilized in biochemical research as a substrate for detecting beta-galactosidase activity. It is a chromogenic substrate that turns yellow upon cleavage by beta-galactosidase, facilitating the detection and quantification of this enzyme in biological samples.
Uses
Used in Biochemical Research:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used as a substrate for detecting beta-galactosidase activity, allowing researchers to monitor the presence and activity of this enzyme in various biological samples.
Used in Gene Expression Studies:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used as a tool in gene expression studies to assess the activity of beta-galactosidase, which can be an indicator of gene expression levels.
Used in Protein Function Analysis:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used as a reagent in protein function analysis to evaluate the activity of beta-galactosidase and its role in various biological processes.
Used in Cell Viability Assessments:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used as an indicator in cell viability assays to determine the health and metabolic activity of cells, as the cleavage of the substrate by beta-galactosidase is a sign of cellular function.
Used in Diagnostic Test Development:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used as a component in the development of diagnostic tests for various diseases and conditions, where the detection of beta-galactosidase activity can be relevant to the diagnosis.
Used in Pharmaceutical Industry:
P-NITROPHENYL 1-THIO-BETA-D-GALACTOPYRANOSIDE is used in the pharmaceutical industry for drug discovery and development, particularly in assays that assess the activity of beta-galactosidase as a target for therapeutic intervention.
Check Digit Verification of cas no
The CAS Registry Mumber 1230-27-9 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,2,3 and 0 respectively; the second part has 2 digits, 2 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 1230-27:
(6*1)+(5*2)+(4*3)+(3*0)+(2*2)+(1*7)=39
39 % 10 = 9
So 1230-27-9 is a valid CAS Registry Number.
InChI:InChI=1/C12H15NO7S/c14-5-8-9(15)10(16)11(17)12(20-8)21-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8?,9-,10+,11-,12+/m1/s1
1230-27-9Relevant articles and documents
Synthesis, biological evaluation, wac and NMR studies of s-galactosides and non-carbohydrate ligands of cholera toxin based on polyhydroxyalkylfuroate moieties
Ramos-Soriano, Javier,Niss, Ulf,Angulo, Jesus,Angulo, Manuel,Moreno-Vargas, Antonio J.,Carmona, Ana T.,Ohlson, Sten,Robina, Inmaculada
, p. 17989 - 18003 (2014/01/17)
The synthesis of several non-carbohydrate ligands of cholera toxin based on polyhydroxyalkylfuroate moieties is reported. Some of them have been linked to D-galactose through a stable and well-tolerated S-glycosidic bond. They represent a novel type of non-hydrolyzable bidentate ligand featuring galactose and polyhydroxyalkylfuroic esters as pharmacophoric residues, thus mimicking the GM1 ganglioside. The affinity of the new compounds towards cholera toxin was measured by weak affinity chromatography (WAC). The interaction of the best candidates with this toxin was also studied by saturation transfer difference NMR experiments, which allowed identification of the binding epitopes of the ligands interacting with the protein. Interestingly, the highest affinity was shown by non-carbohydrate mimics based on a polyhydroxyalkylfuroic ester structure. No carbs here: Saturation transfer difference (STD) NMR studies of bidentate ligands of cholera toxin (see figure, WAC = weak affinity chromatography) show the methylfuran moiety as the main contact point in the interaction with the toxin. Several polyhydroxyalkylfuroate-based structures are synthesized and analyzed and show similar or even better affinity than the bidentate ligands. They constitute the first examples of non-carbohydrate ligands for cholera toxin. Copyright
Mild stereoselective syntheses of thioglycosides under PTC conditions and their use as active and latent glycosyl donors
Cao,Meunier,Andersson,Letellier,Roy
, p. 2303 - 2312 (2007/10/02)
Mild and stereoselective arylthio glycoside syntheses were accomplished by inversion of configuration of glycosyl halides under phase transfer catalyzed conditions. Under such conditions, aryl α-thiosialosides having electron donating and withdrawing subs
