1238-09-1Relevant articles and documents
The molecular basis for the selection of captopril cis and trans conformations by angiotensin I converting enzyme
Tzakos, Andreas G.,Naqvi, Nawazish,Comporozos, Konstantinos,Pierattelli, Roberta,Theodorou, Vassiliki,Husain, Ahsan,Gerothanassis, Ioannis P.
, p. 5084 - 5087 (2006)
Enzyme-inhibitor recognition is considered one of the most fundamental aspects in the area of drug discovery. However, the molecular mechanism of this recognition process (induced fit or prebinding and adaptive selection among multiple conformers) in several cases remains unexplored. In order to shed light toward this step of the recognition process in the case of human angiotensin I converting enzyme (hACE) and its inhibitor captopril, we have established a novel combinatorial approach exploiting solution NMR, flexible docking calculations, mutagenesis, and enzymatic studies. We provide evidence that an equimolar ratio of the cis and trans states of captopril exists in solution and that the enzyme selects only the trans state of the inhibitor that presents architectural and stereoelectronic complementarity with its substrate binding groove.
γ-Valerolactone (GVL): An eco-friendly anchoring solvent for solid-phase peptide synthesis
Al Musaimi, Othman,El-Faham, Ayman,Basso, Alessandra,de la Torre, Beatriz G.,Albericio, Fernando
, (2019/08/26)
Due to the hazardous nature of CH2Cl2, regulatory authorities have imposed restrictions to minimize or even stop its use. It has therefore become imperative to identify environmentally benign solvents to replace it. Here we report on a bio derived solvent, γ-valerolactone, for the incorporation of the first amino acid onto p-alkoxybenzyl alcohol resin in solid-phase peptide synthesis. Satisfactory loading values (by a spectrophotometric method) were achieved. Furthermore, racemization and dipeptide formation were also checked and found to be acceptable.
Trends in asymmetric Michael reactions catalysed by tripeptides in combination with an achiral additive in different solvents
Tsogoeva, Svetlana B.,Jagtap, Sunil B.,Ardemasova, Zoya A.,Kalikhevich, Victor N.
, p. 4014 - 4019 (2007/10/03)
The potential of tripeptides 3, 6 and 12 as chiral catalysts for asymmetric Michael addition reactions in the presence of an achiral additive has been tested in different solvents (CHCI3, acetone, DMF, DMSO and the room-temperature ionic liquid
