127861-47-6Relevant articles and documents
NMR fingerprints of the drug-like natural-product space identify iotrochotazine a: A chemical probe to study Parkinson's disease
Grkovic, Tanja,Pouwer, Rebecca H.,Vial, Marie-Laure,Gambini, Luca,Noel, Alba,Hooper, John N. A.,Wood, Stephen A.,Mellick, George D.,Quinn, Ronald J.
, p. 6070 - 6074 (2014)
The NMR spectrum of a mixture of small molecules is a fingerprint of all of its components. Herein, we present an NMR fingerprint method that takes advantage of the fact that fractions contain simplified NMR profiles, with minimal signal overlap, to allow the identification of unique spectral patterns. The approach is exemplified in the identification of a novel natural product, iotrochotazine A (1), sourced from an Australian marine sponge Iotrochota sp. Compound 1 was used as a chemical probe in a phenotypic assay panel based on human olfactory neurosphere-derived cells (hONS) from idiopathic Parkinson's disease patients. Compound 1 at 1 μM was not cytotoxic but specifically affected the morphology and cellular distribution of lysosomes and early endosomes. Seeing the invisible: A novel natural product, iotrochotazine A was isolated using an NMR-based strategy focused on the analysis of 1H NMR fingerprints of a prefractionated natural-product library. In a phenotypic assay panel based on hONS cells derived from idiopathic Parkinson's disease (PD) patients, it affected the morphology and cellular distribution of lysosomes and early endosomes.
