15313-37-8Relevant articles and documents
Syntheses of [6,7-15N]-adenosine, [6,7-15N]-2'-deoxyadenosine, and [7-15N]-hypoxanthine
Pagano,Lajewski,Jones
, p. 11669 - 11672 (1995)
We have developed a high-yield route for the synthesis of [7-15N]-hypoxanthine in four steps in an overall yield of 81%. This procedure uses [15N]-sodium nitrite as the 15N source and an inexpensive pyrimidine to provide an economical route to this useful 15N-labeled intermediate. Conversion to [7-15N]-6-chloropurine followed by enzymatic transglycosylation gives the corresponding ribo- and 2'-deoxyribonucleosides. Ammonolysis of the 6-chloro moiety to give the [6,7-15N]-labeled nucleosides is effected simply and in high yield using 2 equiv of [15N]-ammonium chloride and 3 equiv of potassium bicarbonate.