1596-56-1 Usage
Description
Naphthol AS-MX Phosphate is a chemical compound that is soluble in dioxane and primarily functions as a substrate for phosphatase enzymes. It is a solid in its chemical form and is widely utilized in various applications due to its unique properties.
Uses
Used in Biomedical Research:
Naphthol AS-MX Phosphate is used as a substrate for alkaline phosphatase in human osteoblasts and mouse embryonic stem cell (mESC) coronal plane sections. This application is crucial for fluorescence staining and analysis, allowing researchers to study the activity and function of alkaline phosphatase in these cell types.
Used in Diagnostics:
In the field of diagnostics, Naphthol AS-MX Phosphate is employed as a component in various assays and tests that require the detection or measurement of phosphatase activity. Its role as a substrate makes it an essential tool for diagnosing and monitoring conditions related to phosphatase enzyme levels.
Used in Pharmaceutical Development:
The compound is also used in the development of new drugs targeting phosphatase enzymes. By understanding the interactions between Naphthol AS-MX Phosphate and these enzymes, researchers can design more effective medications for treating diseases associated with phosphatase dysregulation.
Used in Analytical Chemistry:
In analytical chemistry, Naphthol AS-MX Phosphate is utilized as a reagent for the detection and quantification of phosphatase activity. Its ability to serve as a substrate for these enzymes makes it a valuable tool for researchers working on the characterization and analysis of phosphatase enzymes in various samples.
Used in Education and Training:
Naphthol AS-MX Phosphate is also used in educational settings and training programs to teach students and researchers about the role of phosphatase enzymes in biological systems. By providing a practical, hands-on experience with the compound, learners can gain a deeper understanding of its applications and importance in various fields.
Check Digit Verification of cas no
The CAS Registry Mumber 1596-56-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,5,9 and 6 respectively; the second part has 2 digits, 5 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 1596-56:
(6*1)+(5*5)+(4*9)+(3*6)+(2*5)+(1*6)=101
101 % 10 = 1
So 1596-56-1 is a valid CAS Registry Number.
InChI:InChI=1/C19H18NO5P/c1-12-7-8-17(13(2)9-12)20-19(21)16-10-14-5-3-4-6-15(14)11-18(16)25-26(22,23)24/h3-11H,1-2H3,(H,20,21)(H2,22,23,24)
1596-56-1Relevant articles and documents
Fluorescent substrates for potential use in enzyme-linked immunosorbent assay of membrane-bound nucleic acids
Fujita, Satoshi,Momiyama, Masayoshi,Kondo, Yasumitsu,Kagiyama, Naoto,Hori, Samuel H.,Toru, Takeshi
, p. 1347 - 1353 (2007/10/02)
A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2′-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2′-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of λ DNA can be successfully detected on the membrane with HBNP.
