195385-93-4 Usage
General Description
4-Methylumbelliferylbeta-D-ribofuranoside is a chemical compound that is commonly used in laboratory settings for the detection and measurement of enzymatic activity. It is often used as a substrate to assay the activity of glycosidase enzymes, particularly beta-D-glucosidases and beta-D-galactosidases. The compound is a fluorogenic substrate, meaning that when it is hydrolyzed by an enzyme, it produces a fluorescent product that can be easily detected and quantified. This makes it a valuable tool for studying the kinetics and mechanisms of enzyme-catalyzed reactions, as well as for screening potential inhibitors or activators of glycosidase enzymes. Additionally, 4-Methylumbelliferylbeta-D-ribofuranoside has also been utilized in the study of lysosomal storage disorders, as deficiencies in specific glycosidase enzymes can lead to the accumulation of undigested substrates in the lysosomes of affected cells. Overall, this compound is an important tool for research in biochemistry and enzymology.
Check Digit Verification of cas no
The CAS Registry Mumber 195385-93-4 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,9,5,3,8 and 5 respectively; the second part has 2 digits, 9 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 195385-93:
(8*1)+(7*9)+(6*5)+(5*3)+(4*8)+(3*5)+(2*9)+(1*3)=184
184 % 10 = 4
So 195385-93-4 is a valid CAS Registry Number.
InChI:InChI=1/C15H16O7/c1-7-4-12(17)21-10-5-8(2-3-9(7)10)20-15-14(19)13(18)11(6-16)22-15/h2-5,11,13-16,18-19H,6H2,1H3/t11-,13-,14+,15-/m0/s1
195385-93-4Relevant articles and documents
de Bruyne,Loontiens
, p. 661 (1965)
An improved helferich method for the α/β-stereoselective synthesis of 4-methylumbelliferyl glycosides for the detection of microorganisms
Wei, Xianhu,Ma, Yanxia,Wu, Qingping,Zhang, Jumei,Cai, Zhihe,Lu, Mianfei,Ferro, Vito
, p. 21681 - 21699 (2016/01/25)
An improved Helferich method is presented. It involves the glycosylation of 4-methyl-umbelliferone with glycosyl acetates in the presence of boron trifluoride etherate combined with triethylamine, pyridine, or 4-dimethylaminopyridine under mild conditions, followed by deprotection to give fluorogenic 4-methylumbelliferyl glycoside substrates. Due to the use of base, the glycosylation reaction proceeds more easily, is uncommonly α- or β-stereoselective, and affords the corresponding products in moderate to excellent yields (51%-94%) under appropriate conditions.
Synthesis of 4-methylcoumarin-7-yl β-D-galactofuranoside, a fluorogenic substrate for galactofuranosidase
Marino, Carla,Cancio, Maria J.,Varela, Oscar,Lederkremer, Rosa M. de
, p. 209 - 214 (2007/10/03)
Keywords: 4-Methylcoumarin-7-yl β-D-galactofuranoside; Fluorogenic substrate; Galactofuranosidase
