104-23-4Relevant articles and documents
Rapid and direct spectrophotometric method for kinetics studies and routine assay of peroxidase based on aniline diazo substrates
Mirazizi, Fatemeh,Bahrami, Azita,Haghbeen, Kamahldin,Shahbani Zahiri, Hossein,Bakavoli, Mehdi,Legge, Raymond L.
, p. 1162 - 1169 (2016)
Peroxidases are ubiquitous enzymes that play an important role in living organisms. Current spectrophotometrically based peroxidase assay methods are based on the production of chromophoric substances at the end of the enzymatic reaction. The ambiguity regarding the formation and identity of the final chromophoric product and its possible reactions with other molecules have raised concerns about the accuracy of these methods. This can be of serious concern in inhibition studies. A novel spectrophotometric assay for peroxidase, based on direct measurement of a soluble aniline diazo substrate, is introduced. In addition to the routine assays, this method can be used in comprehensive kinetics studies. 4-[(4-Sulfophenyl)azo]aniline (λ max = 390 nm, ? = 32 880 M?1 cm?1 at pH 4.5 to 9) was introduced for routine assay of peroxidase. This compound is commercially available and is indexed as a food dye. Using this method, a detection limit of 0.05 nmol mL?1 was achieved for peroxidase.
The mechanism of sonophotocatalytic degradation of methyl orange and its products in aqueous solutions
He, Yuanhua,Grieser, Franz,Ashokkumar, Muthupandian
, p. 974 - 980 (2011)
In this study, it was found that a hybrid technique, sonophotocatalysis, is able to degrade a parent organic pollutant (methyl orange) as well as its by-products. The analysis of products formed during the whole degradation has demonstrated that the pH or the selection of oxidation process (sonolysis/photocatalysis/sonophotocatalysis) is able to control the degradation pathway. It was shown in the by-products analysis that the solution pH can alter the amount of each product generated during the sonophotocatalytic degradation. It was revealed that the different degradation rates of methyl orange and its products result from the solution pH and the nature of the organic products. Furthermore, a comparison of the data obtained from the oxidation processes on the degradation of the reaction intermediates identified the advantages of the combined system. It is concluded that sonophotocatalysis is capable of yielding a more complete and faster mineralization of organic pollutants than the individual processes. However, as in the degradation of the parent compound, the overall mineralization is lower than an additive effect (negative synergistic effect).
Preparation of aminoazo dyes
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, (2008/06/13)
Aminoazo dyes are prepared from the corresponding N-sulfomethylated aminoazo dyes by aqueous acid hydrolysis in the presence of amidosulfuric acid, urea or mixtures thereof.
