1132-61-2 Usage
Description
MOPS (3-morpholinopropanesulfonic acid) is a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES. Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.It is used as a synthetic buffering agent below pH 7.5.
application
MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. Usage above 20 mM in mammalian cell culture work is not recommended. MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.
Reference
P. H. Quail, D. Marme, E. Sch?fer, Particle-bound phytochrome from maize and pumpkin, Nature New Biology, 1973, vol. 245, pp. 189-191
Chemical Properties
White/clear crystalline powder
Uses
Different sources of media describe the Uses of 1132-61-2 differently. You can refer to the following data:
1. 3-(N-Morpholino)propanesulfonic acid or MOPS due to its inert nature is a preferred and widely used buffer in many biochemical studies.MOPS has been used as:a cell culture additive component in lentiviral particle production.as a buffering agent in microbial growth medium and nuclei extraction buffer.as a component of Roswell Park Memorial Institute (RPMI) medium for diluting fungal inoculum.as a buffer in capillary-zone electrophoresis to test performance.for the dilution of proteins from algal samples.
2. MOPS acts as a multi-purpose buffering agent used in various biological research.
3. MOPS has been used as:a cell culture additive component in lentiviral particle productionas a buffering agent in microbial growth medium and nuclei extraction buffer
Definition
ChEBI: 3-(N-morpholino)propanesulfonic acid is a Good's buffer substance, pKa = 7.2 at 20 ℃. It is a member of morpholines, a MOPS and an organosulfonic acid. It is a conjugate acid of a 3-(N-morpholino)propanesulfonate. It is a tautomer of a 3-(N-morpholiniumyl)propanesulfonate.
General Description
3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.
Flammability and Explosibility
Notclassified
Check Digit Verification of cas no
The CAS Registry Mumber 1132-61-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 1,1,3 and 2 respectively; the second part has 2 digits, 6 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 1132-61:
(6*1)+(5*1)+(4*3)+(3*2)+(2*6)+(1*1)=42
42 % 10 = 2
So 1132-61-2 is a valid CAS Registry Number.
InChI:InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
1132-61-2Relevant articles and documents
Preparation method of biological buffer agent--moropholino ethanesulfonic acid (MOPS)
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Paragraph 0023-0027; 0030-0033, (2019/07/04)
The invention belongs to the field of organic synthesis and specifically relates to a preparation method of a biological buffer agent--moropholino ethanesulfonic acid (MOPS), comprising the followingstep: morpholine and 1,3-propane sultone (1,3-PS) react to generate moropholino ethanesulfonic acid. The raw materials used in the method are cheaper than raw materials used in the method for synthesizing moropholino ethanesulfonic acid by using 1,3-dibromopropane and morpholine, and the method of the invention is beneficial to large-scale production and application. by using ethanol and the likeas a solvent, the solvent is easy to remove, thus meeting the requirements of users on solvent residue. In the method, a reducing agent is added and nitrogen protection method is adopted for decoloration, thus ensuring chroma of MOPS and making the properties of the product to be better. The preparation process is simple and is convenient to operate and has high practicality.
Aromatic compounds Mannich reaction using economical acidic ionic liquids based on morpholinium salts as dual solvent-catalysts
Yue, Caibo
experimental part, p. 3640 - 3647 (2010/12/25)
Economical acidic ionic liquids containing an alkanesulfonic acid group in a morpholinium cation were found to be effective catalysts for the Mannich reactions of various kinds of aromatic aldehydes, acetophenone and aromatic amines at mild reaction conditions. The satisfactory results were obtained with short reaction time, good yields of the Mannich bases and simplicity in the experimental procedure. These ionic liquids could be recycled and reused for up to five times without considerable decreasing in the catalytic activity. Copyright
Isolation of nucleic acids
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, (2008/06/13)
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind negatively charged nucleic acids and then eluting the nucleic acids at a pH when the said materials possess a neutral or negative charge to release the nucleic acids. The nucleic acids can be removed under mildly alkaline conditions to the maintain integrity of the nucleic acids and to allow retrieval of the nucleic acids in reagents that are immediately compatible with either storage or analytical testing.
