118374-59-7 Usage
Description
CYTIDINE-5'-O-(1-THIOTRIPHOSPHATE), RP-ISOMER SODIUM SALT is a nucleotide analog used in biochemical and molecular biological research, featuring a specific stereochemistry that allows incorporation into RNA during transcription for studying RNA synthesis and processing.
Uses
Used in Biochemical Research:
CYTIDINE-5'-O-(1-THIOTRIPHOSPHATE), RP-ISOMER SODIUM SALT is used as a research tool for investigating RNA synthesis and processing, providing insights into the mechanisms of RNA biology.
Used in Molecular Biology:
In molecular biology, CYTIDINE-5'-O-(1-THIOTRIPHOSPHATE), RP-ISOMER SODIUM SALT is used as a component in experiments aiming to understand the role of RNA in various biological processes, including gene expression and regulation.
Used in Laboratory Settings:
The sodium salt form of CYTIDINE-5'-O-(1-THIOTRIPHOSPHATE), RP-ISOMER is commonly used in laboratories due to its increased stability and solubility, facilitating its application in a wide range of experiments and analyses.
Check Digit Verification of cas no
The CAS Registry Mumber 118374-59-7 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,1,8,3,7 and 4 respectively; the second part has 2 digits, 5 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 118374-59:
(8*1)+(7*1)+(6*8)+(5*3)+(4*7)+(3*4)+(2*5)+(1*9)=137
137 % 10 = 7
So 118374-59-7 is a valid CAS Registry Number.
118374-59-7Relevant articles and documents
Rapid and Efficient Synthesis of Nucleoside 5'-O-(1-Thiotriphosphates), 5'-Triphosphates and 2',3'-Cyclophosphorothioates Using 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one
Ludwig, Janos,Eckstein, Fritz
, p. 631 - 635 (2007/10/02)
2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one phosphitylates the 5'-hydroxy group of a nucleoside to form an intermediate (2), which on subsequent reaction with pyrophosphate produces, in a double displacement process, a P2,P3-dioxo-P1-5'-nucleosidylcyclotriphophite (3).Oxidation with sulfur gives a nucleoside 5'-(1-thiocyclotriphosphate) (4), which is hydrolyzed to the diastereomeric mixture of a nucleoside 5'-O-(1-thiotriphosphate) (5).Alternatively, 3 can be oxidized with iodine/water to furnish nucleoside 5'-triphosphates 6.This reagent can also be applied to the synthesis of nucleoside 2',3'-cyclic phosphorothioates.Protection of nucleobase functional groups is not required.
