1227633-49-9 Usage
Description
SR1 (1227633-49-9)?extends the pluripotency of hematopoietic stem cells by antagonizing the arylhydrocarbon receptor (AhR), IC50?= 127?nM.1?The effect is dose-dependent and reversible. Displays a biphasic effect on CD34-negative cells with low does (1 μM) enhancing proliferation and high does (>5 μM) repressing proliferation.2?May be used to generate progenitor T cells3?from HSPCs as well as NK cells for immuno-oncology studies4.
General Description
A cell-permeable purine compound that acts as a high affinity AhR antagonist (IC50 = 127 nM against 3 nM TCDD-induced luciferase reporter activity in HepG2 cultures), but not a panel of 61 kinases. AhR expression and function knockouts by shRNAs, CH-223191 (Cat. No. 182705), or SR1 treatment all result in enhanced and sustained CD34+ population in long-term proliferating/expanding cultures of cord blood-derived HSCs (hematopoietic setem cells) in serum-free media containing Tpo (Cat. No. 605218), SCF (Cat. No. 569600), Flt3 Ligand (Cat. No. 343010), and IL-6 (Cat. No. 407652) in a reversible manner. SR1 is reactive toward human, monkey, and canine, but not rat or murine, species.
References
1) Boitano?et al.?(2010),?Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells; Science,?329?1354
2) Tao?et al.?(2017),?StemRegenin 1 selectively promotes expansion of Multipotent Hematopoietic Progenitors derived from Human Embryonic Stem Cells; J. Stem Cells Regen. Med.,?13?75
3) Singh?et al.?(2019),?Generation and function of progenitor T cells from stemRegenin-1-expanded CD34+ human hematopoietic progenitor cells;?Blood Adv,?3?2934
4) Hoogstad-van Evert?et al.?(2017),?Umbilical cord blood CD34+ progenitor-derived NK cells efficiently kill ovarian cancer spheroids and intraperitoneal tumors in NOD/SCID/IL2Rgnull?mice; Oncoimmunology,?6(8)?e1320630
Check Digit Verification of cas no
The CAS Registry Mumber 1227633-49-9 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,2,2,7,6,3 and 3 respectively; the second part has 2 digits, 4 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 1227633-49:
(9*1)+(8*2)+(7*2)+(6*7)+(5*6)+(4*3)+(3*3)+(2*4)+(1*9)=149
149 % 10 = 9
So 1227633-49-9 is a valid CAS Registry Number.
1227633-49-9Relevant articles and documents
Methods of Culturing and Expanding Mesenchymal Stem Cells and Isolated Cell Populations Generated Thereby
-
, (2014/02/16)
A method of culturing mesenchymal stem cells (MSCs) is provided. The method comprising culturing a population of the MSCs in a medium comprising an aryl hydrocarbon receptor antagonist, thereby culturing MSCs.