1239020-56-4Relevant articles and documents
A lanthanide-complex-based ratiometric luminescent probe specific for peroxynitrite
Song, Cuihong,Ye, Zhiqiang,Wang, Guilan,Yuan, Jingli,Guan, Yafeng
, p. 6464 - 6472 (2010)
A lanthanide-complex-based ratiometric luminescence probe specific for peroxynitrite (ONOO-), 4'-(2, 4-dimethoxyphenyl)-2,2':6',2 - terpyridine6,6 -diyl]bis(methylenenitrilo)tetrakis(acetate)-Eu 3+/Tb3+ ([Eu3+/Tb3+ (DTTA)]), has been designed and synthesized. Both [EU3+(DTTA)] and [Tb 3+(DTTA)] are highly water soluble with large stability constants at ≈1020, and strongly luminescent with luminescence quantum yields of 10.0 and 9.9%, respectively, and long lumines-cence lifetimes of 1.38 and 0.26 ms, respectively. It was found that the luminescence of [Tb 3+(DTTA)] could be quenched by ONOO- rapidly and specifically in aqueous buffers, while that of [Eu3+(DTTA)] did not respond to the addition of ONOO-. Thus, by simply mixing [Eu 3+(DTTA)] and [Tb3+(DTTA)] in an aqueous buffer, a ratiometric luminescence probe specific for time-gated luminescence detection of ONOO- was obtained. The performance of [Tb3+(DTTA)] and [Eu3+/ Tb3+(DTTA)] as the probes for luminescence imaging detection of ONOO- in living cells was investigated. The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application.
