1241912-74-2 Usage
Description
NTA-FITC, also known as Fluorescein isothiocyanate-Ni2+-nitrilotriacetic acid, is a fluorescent reagent derived from nitriloacetic acid and fluorescein isothiocyanate. It is an orange solid that possesses unique chemical properties, allowing it to bind with high affinity to His-tagged proteins. NTA-FITC is widely used in various applications due to its ability to label and detect His-tagged proteins with high specificity and sensitivity.
Uses
Used in Biochemical Research:
NTA-FITC is used as a fluorescent reagent for the detection of proteins having a Histidine-tag (his-tag). This application is particularly useful in biochemical research, where the identification and quantification of His-tagged proteins are crucial for understanding protein expression, purification, and interaction studies.
Used in Pharmaceutical Industry:
In the pharmaceutical industry, NTA-FITC is used as a tool for drug discovery and development. Its ability to label His-tagged proteins allows researchers to track and analyze the behavior of these proteins in various assays, such as enzyme inhibition, receptor binding, and cell-based assays. This information is vital for the design and optimization of new drug candidates targeting specific proteins.
Used in Diagnostics:
NTA-FITC is also employed in the development of diagnostic tools, where its high specificity for His-tagged proteins can be utilized to detect and quantify specific biomarkers in biological samples. This application is particularly relevant in the field of personalized medicine, where the detection of specific protein biomarkers can aid in the diagnosis and monitoring of various diseases.
Used in Quality Control:
In the biotechnology and pharmaceutical industries, NTA-FITC is used as a quality control tool to ensure the purity and integrity of His-tagged protein products. By labeling these proteins with NTA-FITC, manufacturers can easily monitor the presence of contaminants, aggregation, or misfolding, ensuring that the final product meets the required quality standards.
Check Digit Verification of cas no
The CAS Registry Mumber 1241912-74-2 includes 10 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 7 digits, 1,2,4,1,9,1 and 2 respectively; the second part has 2 digits, 7 and 4 respectively.
Calculate Digit Verification of CAS Registry Number 1241912-74:
(9*1)+(8*2)+(7*4)+(6*1)+(5*9)+(4*1)+(3*2)+(2*7)+(1*4)=132
132 % 10 = 2
So 1241912-74-2 is a valid CAS Registry Number.
1241912-74-2Relevant articles and documents
Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe
Hatai, Joydev,Prasad, Pragati Kishore,Lahav-Mankovski, Naama,Oppenheimer-Low, Noa,Unger, Tamar,Sirkis, Yael Fridmann,Dadosh, Tali,Motiei, Leila,Margulies, David
, p. 1875 - 1878 (2021)
Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in liveEscherichia coli. One of these probes was designed to light up upon binding, which provided the means
