14131-42-1Relevant articles and documents
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Montgomery et al.
, (1943)
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Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan
Ota, Ryo,Okamoto, Yumi,Vavricka, Christopher J.,Oka, Takuji,Matsunaga, Emiko,Takegawa, Kaoru,Kiyota, Hiromasa,Izumi, Minoru
, p. 99 - 103 (2019/01/16)
β-D-Galactofuranose (Galf) is a component of polysaccharides and glycoconjugates. There are few reports about the involvement of galactofuranosyltransferases and galactofuranosidases (Galf-ases) in the synthesis and degradation of galactofuranose-containing glycans. The cell walls of filamentous fungi in the genus Aspergillus include galactofuranose-containing polysaccharides and glycoconjugates, such as O-glycans, N-glycans, and fungal-type galactomannan, which are important for cell wall integrity. In this study, we investigated the synthesis of p-nitrophenyl β-D-galactofuranoside and its disaccharides by chemo-enzymatic methods including use of galactosidase. The key step was selective removal of the concomitant pyranoside by enzymatic hydrolysis to purify p-nitrophenyl β-D-galactofuranoside, a promising substrate for β-D-galactofuranosidase from Streptomyces species.
Substrate and preparation method and application thereof
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, (2019/05/15)
The invention discloses a substrate namely alpha-D-glucoside for detecting activity of alpha-glucosidases, and further discloses a synthesis technology of the substrate. The synthesis technology comprises the steps of enabling a compound alpha-D-glucose and acetyl chloride to be subjected to a reaction, and performing purification to obtain alpha-D- pentacetylglucose; catalyzing the obtained substances with N,N-Dimethyl-1,3-diaminopropane, performing pickling, performing extraction, performing drying, and performing purifying to obtain 2,3,4,6-tetra-O-acetyl-alpha-D-acetyl-glucosamine; catalyzing the obtained substances in a dichloromethane solution, performing a reaction with trichloroacetonitrile, performing filtering, and performing purifying to obtain 2,3,4,6-tetra-O-acetyl-beta-D-glucosyl trichloroacetimidate; performing catalyzing on the obtained substances in a dichloromethane solution, performing a reaction with chromogen or fluorophore, performing extraction, merging organic phases, performing drying, performing concentrating, performing methanol redissolution, performing crystal nourishing at 4 DEG C, and performing filtering to obtain 2,3,4,6-tetra-O-acetyl-alpha-D-glucoside; and catalyzing the obtained substances in a methanolic solution, performing a reaction, then performing decoloring adsorption, performing filtering, and performing purifying. The synthesis technology of the substrate is simple and high in yield, the sensitivity of a reagent kit can be improved, the stability is good, and the specificity is high.