149231-65-2 Usage
General Description
AC-YVAD-AMC (N-Acetyl-Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin) is a sensitive fluorogenic substrate used in scientific research, specifically for studying enzymes. It is predominantly utilized to measure caspase-1 activity, an enzyme associated with programmed cell death. Moreover, the chemical can serve as an important tool in biochemical assays aimed at investigating processes such as apoptosis (cell death) and inflammation. When cleaved by the Caspase-1 enzyme, AC-YVAD-AMC exhibits fluorescence, which facilitates the monitoring and detection of the enzyme's activity. It is important to handle this chemical with care due to its sensitive nature.
Check Digit Verification of cas no
The CAS Registry Mumber 149231-65-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,4,9,2,3 and 1 respectively; the second part has 2 digits, 6 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 149231-65:
(8*1)+(7*4)+(6*9)+(5*2)+(4*3)+(3*1)+(2*6)+(1*5)=132
132 % 10 = 2
So 149231-65-2 is a valid CAS Registry Number.
149231-65-2Relevant articles and documents
A substrate combinatorial array for caspases
Lee, Dennis,Adams, Jerry L.,Brandt, Martin,DeWolf Jr., Walter E.,Keller, Paul M.,Levy, Mark A.
, p. 1667 - 1672 (2007/10/03)
An efficient strategy for the synthesis of a tetrapeptidyl substrate combinatorial array directed toward the caspases is described. Testing of this array with caspases 1 and 4 gave substrate hydrolytic profiles characteristic of each caspase, and permitted the identification of efficiently processed substrates. A comparison of this approach to that using a positional scanning library is presented.
