158442-41-2 Usage
Description
Proteasome Inhibitor I is a pharmaceutical compound that functions by inhibiting the activity of proteasomes, which are protein complexes responsible for the degradation of unneeded and damaged proteins within cells. This inhibition can lead to the accumulation of proteins, ultimately disrupting cellular processes and triggering cell death. Proteasome Inhibitor I has demonstrated potential in the treatment of various diseases, particularly cancer, due to its ability to induce apoptosis and reduce viral replication.
Uses
Used in Cancer Treatment:
Proteasome Inhibitor I is used as an anticancer agent, specifically for the treatment of multiple myeloma, a type of blood cancer. It works by blocking the proteasomes, which are involved in the survival and proliferation of cancer cells. By inhibiting these protein complexes, Proteasome Inhibitor I can effectively induce apoptosis (programmed cell death) in cancer cells, thereby reducing tumor growth and progression.
Additionally, Proteasome Inhibitor I is used as an antiviral agent, particularly in primary effusion lymphoma cells. It has been shown to reduce viral replication, which can be beneficial in managing and treating viral infections associated with certain types of cancer.
Biological Activity
zie(otbu)al-cho (psi)1 have been shown to inhibit the proteasome activities in a variety of cell types.peptide aldehyde, psi (z-ile-glu(otbu)-ala-leu-al), inhibits the proteasome 10-fold better than calpain but is less potent than mg1322. since mg132, psi, mg115 (z-leu-leu-nval-al) and alln can all inhibit calpains and various lysosomal cathepsins in addition to the proteasome, when using these inhibitors in cell culture it is important to perform control experiments to con¢rm that the observed e?ects are due to the inhibition of the proteasome. first, one can use agents, which block intracellular cysteine proteases, but do not inhibit proteasomes3. such inhibitors are z-leu-leu- al, and e-64 for calpains4, and weak bases such as chloroquine and e-64 for lysosomal proteolysis . in yeast, where digestive vacuoles contain mainly serine, not cysteine, proteases, phenylmethylsulfonyl £uoride can be used to inhibit these enzymes without affecting proteasomes5.despite the availability of these inhibitors, mg132, due to its low cost and the rapid reversibility of its action, still remains, in our opinion, the first choice to study proteasome involvement in a process in cell cultures or tissues, if appropriate controls are used. as the most potent and selective of commercially available aldehydes, mg132 is preferable to alln, mg115 (z-leu-leu-nval-al), or even psi. on the other hand, the least selective inhibitor, alln, because of its ability to inhibit most major pro teases in mammalian cells, is probably the best tool for prevention of unwanted proteolysis, for example during isolation of proteins from mammalian cells.
references
1. takada k (1995) mol. biol. rep. 21: 21–26 2. a. f. kisselev, a. l. goldberg. proteasome inhibitors: from research tools to drug candidates. chemistry & biology 8 (2001) 739-758. 3. w. matthews, j. driscoll, k. tanaka, a. ichihara, a.l. goldberg, involvement of the proteasome in various degradative processes in mammalian cells, proc. natl. acad. sci. usa 86 (1989) 2597-2601. 4. s. tsubuki, y. saito, m. tomioka, h. ito, s. kawashima, differential inhibition of calpain and proteasome activities by peptidyl aldehydes of di-leucine and tri-leucine, j. biochem. 119 (1996) 572-576. 5. d.h. lee, a.l. goldberg, selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in saccharomyces cerevisiae, j. biol. chem. 271 (1996) 27280-27284.
Check Digit Verification of cas no
The CAS Registry Mumber 158442-41-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,5,8,4,4 and 2 respectively; the second part has 2 digits, 4 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 158442-41:
(8*1)+(7*5)+(6*8)+(5*4)+(4*4)+(3*2)+(2*4)+(1*1)=142
142 % 10 = 2
So 158442-41-2 is a valid CAS Registry Number.