1607445-59-9

Basic information

• Product Name: C₆₃H₇₀N₆O₈PS⁽¹⁺⁾
• Synonyms: C₆₃H₇₀N₆O₈PS⁽¹⁺⁾
• CAS NO: 1607445-59-9
• Molecular Weight: 1102.32
• Mol File: 1607445-59-9.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: C₆₃H₇₀N₆O₈PS⁽¹⁺⁾(CAS DataBase Reference)
• NIST Chemistry Reference: C₆₃H₇₀N₆O₈PS⁽¹⁺⁾(1607445-59-9)
• EPA Substance Registry System: C₆₃H₇₀N₆O₈PS⁽¹⁺⁾(1607445-59-9)

Safety Data

• Hazardous Substances Data: 1607445-59-9(Hazardous Substances Data)

Upstream product

• 1607445-56-6 trans-4-methyl-N-(hexene-1-ylboronic acid pinacol ester)quinolinium chloride 
• 197313-32-9 (E)-2-(6-chlorohex-1-en-1-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane 
• 1607445-57-7 5-(trans-4-methyl-N-(hexen-1-yl-)quinolinium)-5'-DMT-uridine 
• 491-35-0 C₆H₄NCH₂CHCCH₂ 
• 1607445-58-8 C₅₄H₅₃N₄O₇S⁽¹⁺⁾ 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 

Relevant articles and documents

Detection of cyclin D1 mRNA by hybridization sensitive NIC-oligonucleotide probe

A large group of fluorescent hybridization probes, includes intercalating dyes for example thiazole orange (TO). Usually TO is coupled to nucleic acids post-synthetically which severely limits its use. Here, we have developed a phosphoramidite monomer, 10, and prepared a 2′-OMe-RNA probe, labeled with 5-(trans-N-hexen-1-yl-)-TO-2′-deoxy-uridine nucleoside, dUTO, (Nucleoside bearing an Inter-Calating moiety, NIC), for selective mRNA detection. We investigated a series of 15-mer 2′-OMe-RNA probes, targeting the cyclin D1 mRNA, containing one or several dUTO at various positions. dUTO-2′-OMe-RNA exhibited up to 7-fold enhancement of TO emission intensity upon hybridization with the complementary RNA versus that of the oligomer alone. This NIC-probe was applied for the specific detection of a very small amount of a breast cancer marker, cyclin D1 mRNA, in total RNA extract from cancerous cells (250 ng/μl). Furthermore, this NIC-probe was found to be superior to our related NIF (Nucleoside with Intrinsic Fluorescence)-probe which could detect cyclin D1 mRNA target only at high concentrations (1840 ng/μl). Additionally, dUT can be used as a monomer in solid-phase oligonucleotide synthesis, thus avoiding the need for post-synthetic modification of oligonucleotide probes. Hence, we propose dU TO oligonucleotides, as hybridization probes for the detection of specific RNA in homogeneous solutions and for the diagnosis of breast cancer.